AAM-CYT-3-4 | Mouse Cytokine Array C3 Clinisciences

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Mouse Cytokine Array C3

RayBio^® C-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.

Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Mouse
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	62
Recommended Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Axl Gene Symbol: Axl Accession #: Q00993 ELISA Kit: RayBio Mouse Axl ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Accession #: P32972 ELISA Kit: RayBio Mouse CD30 L ELISA	CD30 (TNFRSF8) Gene Symbol: CD30 Accession #: Q60846 ELISA Kit: RayBio Mouse CD30 ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Accession #: Q99NE2 ELISA Kit: RayBio Mouse CD40 ELISA
CRG-2 Gene Symbol: Cxcl10 Accession #: P17515 ELISA Kit: RayBio Mouse CRG-2 ELISA	CTACK (CCL27) Gene Symbol: CCL27 Accession #: Q9Z1X0 ELISA Kit: RayBio Mouse CTACK ELISA	CXCL16 Gene Symbol: CXCL16 Accession #: Q5F2D5 ELISA Kit: RayBio Mouse CXCL16 ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA
Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA	GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA
IGFBP-3 Gene Symbol: IGFBP3 Accession #: P47878 ELISA Kit: RayBio Mouse IGFBP-3 ELISA	IGFBP-5 Gene Symbol: IGFBP5 Accession #: Q07079 ELISA Kit: RayBio Mouse IGFBP-5 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Accession #: Q91X24 ELISA Kit: RayBio Mouse IGFBP-6 ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA
IL-12 p40/p70 Gene Symbol: IL12A; IL12B Accession #: P43432 ELISA Kit: RayBio Mouse IL-12 p40/70 ELISA	IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA	IL-17A Gene Symbol: IL17A Accession #: Q60971	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA
IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA	IL-3 R beta Gene Symbol: Csf2rb Accession #: P26955 ELISA Kit: RayBio Mouse IL-3 R beta ELISA	IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-5 Gene Symbol: IL5 Accession #: P04401 ELISA Kit: RayBio Mouse IL-5 ELISA
IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA	IL-9 Gene Symbol: IL9 Accession #: P15247 ELISA Kit: RayBio Mouse IL-9 ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA	Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA	Leptin R Gene Symbol: LEPR Accession #: Q61215 ELISA Kit: RayBio Mouse Leptin R ELISA
LIX Gene Symbol: Cxcl5 Accession #: P50228 ELISA Kit: RayBio Mouse LIX ELISA	L-Selectin (CD62L) Gene Symbol: SELL Accession #: Q3TCF3 ELISA Kit: RayBio Mouse L-Selectin ELISA	Lymphotactin (XCL1) Gene Symbol: XCL1 Accession #: P47993 ELISA Kit: RayBio Mouse Lymphotactin ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	MCP-5 Gene Symbol: Ccl12 Accession #: Q9QYD6 ELISA Kit: RayBio Mouse MCP-5 ELISA
M-CSF Gene Symbol: CSF1 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA	MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	MIP-2 Gene Symbol: Cxcl2 Accession #: P10889 ELISA Kit: RayBio Mouse MIP-2 ELISA
MIP-3 beta (CCL19) Gene Symbol: CCL19 Accession #: O70460 ELISA Kit: RayBio Mouse MIP-3 beta ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Accession #: O89093 ELISA Kit: RayBio Mouse MIP-3 alpha ELISA	Platelet Factor 4 (CXCL4) Gene Symbol: PF4, CXCL4, SCYB4 Accession #: Q9Z126 ELISA Kit: RayBio Mouse PF-4 ELISA	P-Selectin Gene Symbol: SELP Accession #: Q01102 ELISA Kit: RayBio Mouse P-Selectin ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA
SCF Gene Symbol: KITLG Accession #: P20826 ELISA Kit: RayBio Mouse SCF ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Accession #: P40224 ELISA Kit: RayBio Mouse SDF-1 alpha ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Accession #: O88734 ELISA Kit: RayBio Mouse sTNFRII ELISA	TARC (CCL17) Gene Symbol: CCL17 Accession #: Q9WUZ6 ELISA Kit: RayBio Mouse TARC ELISA
I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Accession #: P14098 ELISA Kit: RayBio Mouse TCA-3 ELISA	TECK (CCL25) Gene Symbol: CCL25 Accession #: O35903 ELISA Kit: RayBio Mouse TECK ELISA	TIMP-1 Gene Symbol: TIMP1 Accession #: P12032 ELISA Kit: RayBio Mouse TIMP-1 ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Accession #: P40226 ELISA Kit: RayBio Mouse TPO ELISA
VCAM-1 (CD106) Gene Symbol: VCAM1 Accession #: P29533 ELISA Kit: RayBio Mouse VCAM-1 ELISA	VEGF-A Gene Symbol: VEGFA Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF-A ELISA

Typical Data

AAM-CYT-3 was used to profile plasma cytokines and chemokines in aged wild-type mice following poly(I:C) stimulation, with or without long-term stavudine treatment, revealing reduced inflammatory cytokine responses with stavudine. Figure adapted from Simon et al., Cell Metabolism, 2019.

AAM-CYT-3 was used to profile secreted cytokines in conditioned media from metastatic esophageal squamous cell carcinoma cells, identifying increased Csf-1 secretion associated with Trp53-R172H-driven metastasis. Figure adapted from Efe et al., Cancer Discovery, 2023.

AAM-CYT-3 was used to profile cytokines secreted by ex vivo KrasG12D and KrasWT pancreatic acinar cells, identifying increased G-CSF secretion associated with KrasG12D-driven acinar-to-ductal metaplasia. Figure adapted from Li et al., Developmental Cell, 2024.

AAM-CYT-3 was used to profile 42 cytokines and chemokines in plasma from a representative healthy control and a septic patient at the time of clinical diagnosis (0 h), illustrating early inflammatory mediator changes during sepsis. Figure adapted from Zhu et al., Science Advances, 2023.

AAM-CYT-3 was used to profile serum cytokines in control and CLP-induced septic mice with or without progesterone-entrapping DM-β-cyclodextrin nanoparticle treatment, showing reduced inflammatory signals following therapy. Figure adapted from Qiang et al., Frontiers in Immunology, 2024.

Kit Components

Mouse Cytokine Antibody Array C3 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

Datasheet (AAM-CYT-3.pdf)

SDS (AAM-CYT-3.pdf)

Excel (AAM-CYT-3_Analysis_Tool.xls)

citations

LINE1 Derepression in Aged Wild-Type and SIRT6-Deficient Mice Drives Inflammation
Species:
Mouse
Sample Type:
Plasma (nucleoside reverse-transcriptase inhibitors (D4T treatment))
Year:
2019
Journal Name:
Cell Metabolism
PubMed ID:
30853213
Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure
Species:
Mouse
Sample Type:
Conditioned Media (Mouse Skin Drosal cells Melanocyte Stem cell translocation media)
Year:
2017
Journal Name:
Cell Stem Cell
PubMed ID:
29033353
Resolution of MoS2 Nanosheets-Induced Pulmonary Inflammation Driven by Nanoscale Intracellular Transformation and Extracellular-Vesicle Shuttles
Species:
Mouse
Sample Type:
bronchoalveolar lavage fluid (BALF)
Year:
2023
Journal Name:
Advanced Materials
Efe G, Dunbar KJ, Sugiura K, Cunningham K, Carcamo S, Karaiskos S, Tang Q, Cruz-Acuña R, Resnick-Silverman L, Peura J, Lu C, Hasson D, Klein-Szanto AJ, Taylor AM, Manfredi JJ, Prives C, Rustgi AK
Year:
2023
Journal Name:
Cancer Discovery
PubMed ID:
37676642
Pleiotrophin drives a prometastatic immune niche in breast cancer
Species:
Mouse
Sample Type:
Met-1 cell-Xenograft, 4T1 cell-Xenograft
Year:
2023
Journal Name:
Journal of Experimental Medicine
FRA1 controls acinar cell plasticity during murine KrasG12D-induced pancreatic acinar to ductal metaplasia
Year:
2024
Journal Name:
Developmental Cell
PubMed ID:
39178842
A synthetic metastatic niche reveals antitumor neutrophils drive breast cancer metastatic dormancy in the lungs
Species:
Mouse
Sample Type:
4T1 cell
Year:
2023
Journal Name:
Nature Communications
Identification of procathepsin L (pCTS-L)–neutralizing monoclonal antibodies to treat potentially lethal sepsis
Species:
Mouse
Sample Type:
Serum (macrophage)
Year:
2023
Journal Name:
Science advances
Pancreatic cancer cell-intrinsic transglutaminase-2 promotes T cell suppression through microtubule-dependent secretion of immunosuppressive cytokines
Year:
2025
Journal Name:
J Immunother Cancer
PubMed ID:
39824529
Ferritin-mediated mitochondrial iron homeostasis is essential for the survival of hematopoietic stem cells and leukemic stem cells
Year:
2024
Journal Name:
Leukemia
PubMed ID:
38402368

Mouse Cytokine Array C3

Cat# AAM-CYT-3-4

Brand : RayBiotech

Mouse Cytokine Array C3

Product Description

Specifications

Product Features

Target Names

Typical Data

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

documents

citations

LINE1 Derepression in Aged Wild-Type and SIRT6-Deficient Mice Drives Inflammation

Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure

Resolution of MoS2 Nanosheets-Induced Pulmonary Inflammation Driven by Nanoscale Intracellular Transformation and Extracellular-Vesicle Shuttles

Efe G, Dunbar KJ, Sugiura K, Cunningham K, Carcamo S, Karaiskos S, Tang Q, Cruz-Acuña R, Resnick-Silverman L, Peura J, Lu C, Hasson D, Klein-Szanto AJ, Taylor AM, Manfredi JJ, Prives C, Rustgi AK

Pleiotrophin drives a prometastatic immune niche in breast cancer

FRA1 controls acinar cell plasticity during murine KrasG12D-induced pancreatic acinar to ductal metaplasia

A synthetic metastatic niche reveals antitumor neutrophils drive breast cancer metastatic dormancy in the lungs

Identification of procathepsin L (pCTS-L)–neutralizing monoclonal antibodies to treat potentially lethal sepsis

Pancreatic cancer cell-intrinsic transglutaminase-2 promotes T cell suppression through microtubule-dependent secretion of immunosuppressive cytokines

Ferritin-mediated mitochondrial iron homeostasis is essential for the survival of hematopoietic stem cells and leukemic stem cells

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