Amount Used: ? 0.2 Ug/106 cells BALB/c splenocytes were double-stained with rat anti-mouse λ-R-PE and rat anti-mouse CD19-FITC. Small lymphocytes were then gated and analyzed using a FACScan flow cytometer (BDIS, San Jose, CA).
To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry and/or ELISAto conform to characteristics of a standard reference reagent. Representative data are included in this productinsert.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Identification and enumeration of Lambda+ cells by flow cytometry, Identification and enumeration of Lambda+ cells by immunofluorescence microscopy, Enzyme-Linked-Immunosorbent-Assay (ELISA)
Other Applications Data
Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds.
Other Applications Image 1 Data
Amount Used: ≤ 0.2 Ug/106 cells BALB/c splenocytes were double-stained with rat anti-mouse lamda-R-PE and rat anti-mouse CD19-FITC. Small lymphocytes were then gated and analyzed using a FACScan flow cytometer (BDIS, San Jose, CA).
Storage
- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected.
Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus from the C57BL/6J inbred mouse strain. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. This locus has four J-C clusters and three V segments that constitute two transcriptional units. One of the J-C clusters is incapable of encoding a protein; the J and C gene segments in this cluster are considered pseudogenes. Inbred strains of laboratory mice lack an additional J-C cluster of pseudogenes and several additional V segments that are found in wild mice.
What is the species homology for "Mouse Lambda Antibody : PE (OASB00208)"?
The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".
How long will it take to receive "Mouse Lambda Antibody : PE (OASB00208)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
What buffer format is "Mouse Lambda Antibody : PE (OASB00208)" provided in?
This item is provided in "". Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Mouse Lambda Antibody : PE (OASB00208)"?
This target may also be called "Igl-x" in publications.
What is the shipping cost for "Mouse Lambda Antibody : PE (OASB00208)"?
The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.
What is the guarantee for "Mouse Lambda Antibody : PE (OASB00208)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Mouse Lambda Antibody : PE (OASB00208)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "". Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Mouse Lambda Antibody : PE (OASB00208)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "IGL"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "IGL"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "IGL"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "IGL"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "IGL"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "IGL"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
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