Product Info Summary
| Catalog: | MEK1016 |
|---|---|
| Species: | Mouse |
| Sample Type: | Serum, Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 5.5 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Manufacturer Reference Number: | 111149MS |
| Analytes: | IFN-γ, IL-1β, IL-6, TNFα |
Product info
Product Overview
The Multiplex Mouse Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IFNγ, IL-1β, IL-6, TNFα).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 5.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
Assay Ranges
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IFNγ | Sandwich | 516 - 0.71 (pg/mL) | 595 (pg/mL) | 1.52 (pg/mL) | 0.365 (pg/mL) | 3.3% | 5.5% | 110% |
| IL-1β | Sandwich | 2,500 - 3.43 (pg/mL) | 1,673 (pg/mL) | 4.26 (pg/mL) | 1.71 (pg/mL) | 3.1% | 9.3% | 81% |
| IL-6 | Sandwich | 504 - 0.69 (pg/mL) | 819 (pg/mL) | 2.09 (pg/mL) | 0.35 (pg/mL) | 7.3% | 6.1% | 106% |
| TNFα | Sandwich | 429 - 0.59 (pg/mL) | 217 (pg/mL) | 0.55 (pg/mL) | 0.29 (pg/mL) | 6.9% | 5.2% | 104% |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3.5 mL/vial
Substrate B: Liquid, 3.5 mL/vial
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips
Question
Has MEK1016 been tested with mouse serum sample or other cell line?
Verified customer
Asked: 2021-01-28
Answer
For the Multiplex ELISA Kit For Mouse Cytokine Panel 2 (4-Plex) (MEK1016), we likely tested B6 mouse cell line samples in development years ago, but we do not have specific data to share using these samples.
Boster Scientific Support
Answered: 2021-02-01