NAD/NADH Assay Kit

Principle of the Assay: Nicotinamide adenine dinucleotide (NAD) is a coenzyme found in all cells, including two forms, NAD+(oxidized) and NADH (reduced). NAD+ is not only a coenzyme that transfers electrons during REDOX reaction, but also a substrate for many enzymes to participate in intracellular reactions. NAD+ plays an important role in cells and in vivo. Its synthesis, degradation and its products are participated in apoptosis, metabolism regulation and gene expression regulation, and the reduction of NAD+ is one of the main factors of cell death. The importance of NAD+ in regulating the REDOX state of cells and its function in regulating signaling pathways and transcription make NAD+, its synthesis and consumption enzymes to be potential drug targets for a variety of diseases. CheKine™ CoenzymeI NAD(H) Colorimetric Assay Kit is a WST-8 based chromogenic reaction that uses colorimetry to measure the amount, ratio, and total amount of NAD+(oxidized coenzyme I) and NADH(reduced Coenzyme I) in cells, tissues, or other samples. The NAD+/NADH assay kit is based on an enzyme cycle reaction (which does not recognize NADP+/NADPH) in which NAD+ is reduced to NADH and NADH reduces WST-8 to orange formazan with a maximum absorption peak detected at around 450 nm. The formation of Formazan in the reaction system is proportional to the total amount of NAD+ or NADH in the sample.

Small volumes of NAD/NADH assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9711519 is an assay kit targeting NAD/NADH.