Animal Free, Chromatographically purified. A lyophilized powder.
Neutral Protease (Dispase), Purified
Neutral Protease (Dispase), Purified
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Product Description
Specifications
| Size | 10 mg, 50 mg, 250 mg |
| Accession Number | P29148 |
| Expression System | Bacillus polymyxa |
| Molecular Weight (kDa) | 32.5 kDa |
| Format | Lyophilized (powder) |
| Activity | ≥4 units per mg dry weight |
| Recommended Applications | Tissue disaggregation and subcultivation Prevention of unwanted cell clumping Preparation of cells for culture Separation of intact epidermis from dermis and intact epithelial sheet in culture from the substratum (Kurt et al. 1989) Harvest and transfer of, Tissue disaggregation and subcultivation Prevention of unwanted cell clumping Preparation of cells for culture Separation of intact epidermis from dermis and intact epithelial sheet in culture from the substratum (Kurt et al. 1989) Harvest and transfer of normal, diploid cells and cell lines (Matsumura et. al 1975) Gentle and intact detachment of epidermal cells (Kitano and Okada 1983) |
| Source | Bacillus polymyxa |
| Shipping Type | Ambient temperature |
| Storage | 2-8°C |
Other Information
Extinction Coefficient
45,350 cm-1 M-1 (Theoretical) E1%,280 Equals 13.96 (Theoretical)
Isoelectric
5.14 (Theoretical)
CATH
Class: The protein consists of two distinct domains: the C-terminal is mainly alpha, while the N-terminal is mainly beta. Architecture: The distinct architectures of the C-terminal and N-terminal are orthogonal bundle and roll, respectively. Topology: The C-terminal domain is elastase-like and the N-terminal domain is neutral protease-like.
CAS Registry Number
C.A.S.: 9001-92-7
Storage Conditions
Store at 2-8°C.