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> p53 Antibody

p53 Antibody

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Cat# ASM10126-A680-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • IHC - p53 Antibody ASM10126
    Immunohistochemistry analysis using Mouse Anti-p53 Monoclonal Antibody, Clone Pab1801 (ASM10126). Tissue: esophagus cancer cells. Species: Human. Primary Antibody: Mouse Anti-p53 Monoclonal Antibody (ASM10126) at 1:50000. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain.
  • WB - p53 Antibody ASM10126
    Western Blot analysis of Human HeLa cell lysates showing detection of p53 protein using Mouse Anti-p53 Monoclonal Antibody, Clone Pab1801 (ASM10126). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p53 Monoclonal Antibody (ASM10126) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E, IP, RIA
Primary Accession P04637
Other Accession NP_000537.3
Host Mouse
Isotype IgG1
Reactivity Human
Clonality Monoclonal
Description Mouse Anti-Human p53 Monoclonal IgG1
Target/Specificity Detects ~53kDa.
Other Names P53 Antibody, LFS1 Antibody, TRP53 Antibody, FLJ92943 Antibody, TP53 Antibody, p53 Tumor Suppressor Antibody, Phosphoprotein p53 Antibody, TP53 Antibody, Transformation related protein 53 Antibody, TRP53 Antibody, Tumor protein p53 Antibody
Clone Names Pab 1801
Immunogen Fusion protein (Human), amino acids 46-53
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-183 was sufficient for detection of p53 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus | PML body | Endoplasmic Reticulum