PCNA (PC10)
Cat# sc-56
Size : 200µg/ml
Brand : Santa Cruz Biotechnology
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Anticorps PCNA (PC10): sc-56
- L'anticorps PCNA PC10 est un monoclonal IgG2a κ L'Anticorps PCNA, cité dans 2.910 publications, fourni en 200 µg/ml
- raised against rat PCNA made in the protein A expression vector pR1T2T
- L'Anticorps PCNA (PC10) est recommandé pour la détection de PCNA p36 protein expressed at high levels in proliferating cells d'origine mouse, rat, human, insect et S. pombe par WB, IP, IF, IHC(P) et FCM
- Anti-L'Anticorps PCNA (PC10) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
- aussi disponible conjugué à la biotine pour WB, IHC(P) et ELISA; et soit au TRITC ou Alexa Fluor® 405 pour IF, IHC(P) et FCM
- m-IgG Fc BP-HRP, 2a BP-HRP">m-IgG2a BP-HRP et m-IgGκ BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps PCNA (PC10) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps PCNA (PC10) ().
ACCÈS RAPIDE AUX LIENS
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PCNA Antibody (PC10) is an IgG2a κ mouse monoclonal PCNA antibody (also designated ATLD2 antibody) that detects the PCNA protein of m, r, h, insect, S. pombe origin by WB, IP, IF, IHC(P) and FCM. PCNA Antibody (PC10) is available as both the non-conjugated anti-PCNA antibody form, as well as multiple conjugated forms of anti-PCNA antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The PCNA (proliferating cell nuclear antigen) protein is synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, the PCNA protein exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. Thus, in staining applications, the PCNA Antibody exhibits nuclear staining. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives. Thus, only the synthesizing form is detectable by the PCNA antibody using this method.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- The contribution of E2F-regulated transcription to Drosophila PCNA gene function. | Thacker, SA., et al. 2003. Curr Biol. 13: 53-8. PMID: 12526745
- Germline mutations at microsatellite loci in homozygous and heterozygous mutants for mismatch repair and PCNA genes in Drosophila. | Baida, A., et al. 2003. DNA Repair (Amst). 2: 827-33. PMID: 12826283
- The human proliferating Cell nuclear antigen regulates transcriptional coactivator p300 activity and promotes transcriptional repression. | Hong, R. and Chakravarti, D. 2003. J Biol Chem. 278: 44505-13. PMID: 12937166
- Armadillo/Pangolin regulates PCNA and DREF promoter activities. | Kwon, E., et al. 2004. Biochim Biophys Acta. 1679: 256-62. PMID: 15358517
- GFP-PCNA as an S-phase marker in embryos during the first and subsequent cell cycles. | Kisielewska, J., et al. 2005. Biol Cell. 97: 221-9. PMID: 15584900
- Germline genomic instability in PCNA mutants of Drosophila: DNA fingerprinting and microsatellite analysis. | López, A., et al. 2005. Mutat Res. 570: 253-65. PMID: 15708584
- The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis. | Woods, AL., et al. 1991. Histopathology. 19: 21-7. PMID: 1680784
- Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. | Waseem, NH. and Lane, DP. 1990. J Cell Sci. 96 (Pt 1): 121-9. PMID: 1695635
- Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites. | Bravo, R. and Macdonald-Bravo, H. 1987. J Cell Biol. 105: 1549-54. PMID: 2889739
Espèces | Nom du Gène | Gene ID | Localisation chromosomique | Numéro d'accession de l'isoforme (ARNm) | Numéro d'accès aux protéines | Numéro OMIM™ |
---|---|---|---|---|---|---|
Human | PCNA | Entrez Gene ID: 5111 | 20p13 | NM_002592, NM_182649 | UniProt ID: P12004 | 176740 |
Mouse | Pcna | Entrez Gene ID: 18538 | 2 F2 | NM_011045 | UniProt ID: P17918 | N/A |
Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.
Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PCNA Antibody (PC10) has 2,909 citations in a variety of scientific publications.
Primary antibodies like Anti-PCNA Antibody (PC10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.
What blocking procedure to you recommend for using this mouse monoclonal antibody on mouse tissue?
does antibody m-IgGκ BP-HRP:(sc-516102) compatible to using as secondary antibody against Anti-PCNA Antibody (PC10): sc-56 for western blot
Recently we observed that the PCNA sc-56 antibody in the tube is precipitated. It was stored at 4 degrees as recommended. Can the antibody be used for experimental purpose? If yes how do we use it? any recommendation from company's side?
Which antigen retrieval buffer should be used with PCNA sc-56, citrate or Tris? Can you please include this information in the product datasheet so that it becomes convenient for the user to begin with.
Can I use this mouse monoclonal antibody for immunohistochemical staining of swine(pig) tissue?
We are new to IHC. Thinking of using this antibody, PCNA Antibody: sc-56on our chicken samples. Wondering if there are any preferences on the conjugate for the secondary antibody, such as HRP, biotin etc. We don't need fluorescence
PCNA Antibody (PC10) how much of 200ug/ml of this antibody I am going to get in one order?
I'm wondering about the region of PCNA that is recognized by this antibody SC-56, PC10? Would an N-terminal Flag tag get in the way? Thank you.
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