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> Phalloidin, CF®633

Phalloidin, CF®633

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Cat# 00046-T

Size : 50U

Brand : Biotium

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Phone : +1 850 650 7790

Phalloidin Conjugates

A highly selective actin filament stain for fixed and permeabilized cells. Labeled phalloidins have similar affinity for both large and small filaments.

Phalloidin Conjugates
Phalloidin Conjugates

Product Attributes

Probe cellular localization

Cytoskeleton, F-Actin
For live or fixed cells

For fixed cells
Assay type/options

Tissue staining
Detection method/readout

Fluorescence microscopy
Cell permeability

Membrane impermeant
Fixation options

Permeabilize before staining, Fix before staining (formaldehyde)
Toxin

Phalloidin
Colors

Blue, Green, Orange, Red, Far-red, Near-infrared
Protocols
SDS
Supporting Documents

Product Description

A toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. When conjugated to a fluorescent label, it will stain actin filaments in fixed and permeabilized cells.

  • Choice of 17 bright & photostable CF® Dyes, biotin, or other common fluorophores
  • Dye options for super-resolution and 2-photon imaging
  • Excellent signal to background with negligible non-specific staining

Fluorescently-labeled phalloidins bind to F-actin with nanomolar affinity. Labeled phalloidins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and nonmuscle cells from various species of plants, animals and fungi. Different from antibodies, the binding affinity of phalloidin does not change significantly with actin among different species.

Fluorescently-labeled phalloidin is typically used to stain fixed and permeabilized cells. Labeled phalloidins are not cell-permeant and have therefore not been used extensively with living cells, however they can be loaded into live cells via cationic liposomes.

Note: Phalloidin stock solutions are very stable. But binding of fluorescent phalloidins to cells is more labile than other probes, such as antibodies, and staining can become non-specific or lost over time. For best results, store phalloidin-stained samples in a suitable mounting medium at 4°C, protected from light. For certain phalloidin conjugates, especially CF®405M, CF®647, and CF®680, we recommend imaging immediately or shortly after staining. Staining with our other CF® Dye phalloidins usually is stable for up to a week when specimens are stored at 4°C, protected from light.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

Super-Resolution Microscopy

CF®647 and CF®680 phalloidins are recommended mainly for STORM applications. Certain CF® Dyes are compatible with various super resolution imaging techniques. The superior brightness, photostability, and photochemical switching properties of certain CF® Dyes are ideal for 3-D SIM, 3-D STORM, and other super-resolution and single-molecule imaging approaches. Learn more about CF® Dyes for super-resolution microscopy.

Phalloidin Conjugates

Product Conjugation Ex/Em Size Catalog No. Purchase
Biotin-XX Phalloidin Biotin-XX N/A 100 U 00028 Purchase 00028
CF®350 Phalloidin CF®350 347/448 nm 50 U 00049-T Purchase 00049-T
300 U 00049 Purchase 00049
CF®405M Phalloidin CF®405M 408/452 nm 50 U 00034-T Purchase 00034-T
300 U 00034 Purchase 00034
CF®430 Phalloidin CF®430 426/498 nm 50 U 00054-T Purchase 00054-T
300 U 00054 Purchase 00054
CF®440 Phalloidin CF®440 440/515 nm 50 U 00055-T Purchase 00055-T
300 U 00055 Purchase 00055
CF®488A Phalloidin CF®488A 490/515 nm 50 U 00042-T Purchase 00042-T
300 U 00042 Purchase 00042
CF®532 Phalloidin CF®532 527/558 nm 50 U 00051-T Purchase 00051-T
300 U 00051 Purchase 00051
CF®543 Phalloidin CF®543 541/560 nm 50 U 00043-T Purchase 00043-T
300 U 00043 Purchase 00043
CF®568 Phalloidin CF®568 562/583 nm 50 U 00044-T Purchase 00044-T
300 U 00044 Purchase 00044
CF®583R Phalloidin CF®583R 586/609 nm 50 U 00064-T Purchase 00064-T
300 U 00064 Purchase 00064
CF®594 Phalloidin CF®594 593/614 nm 50 U 00045-T Purchase 00045-T
300 U 00045 Purchase 00045
CF®633 Phalloidin CF®633 630/650 nm 50 U 00046-T Purchase 00046-T
300 U 00046 Purchase 00046
CF®640R Phalloidin CF®640R 642/662 nm 50 U 00050-T Purchase 00050-T
300 U 00050 Purchase 00050
CF®647 Phalloidin* CF®647 650/665 nm 50 U 00041-T Purchase 00041-T*
300 U 00041 Purchase 00041*
CF®660C Phalloidin CF®660C 667/685 nm 50 U 00052-T Purchase 00052-T
300 U 00052 Purchase 00052
CF®660R Phalloidin CF®660R 663/682 nm 50 U 00047-T Purchase 00047-T
300 U 00047 Purchase 00047
CF®680 Phalloidin* CF®680 681/698 nm 50 U 00053-T Purchase 00053-T*
300 U 00053 Purchase 00053*
CF®680R Phalloidin CF®680R 680/701 nm 50 U 00048-T Purchase 00048-T
300 U 00048 Purchase 00048
Fluorescein Phalloidin Fluorescein 496/516 nm 300 U 00030 Purchase 00030
Rhodamine Phalloidin Rhodamine 540/565 nm 300 U 00027 Purchase 00027
Rhodamine 110 Phalloidin Rhodamine 110 502/524 nm 300 U 00032 Purchase 00032
Sulforhodamine 101 (Texas Red®) Phalloidin Texas Red® 591/608 nm 300 U 00033 Purchase 00033
*CF®647 and CF®680 phalloidins are recommended for STORM applications, but due to the instability of staining with these conjugates, we do not recommend using them for other microscopy applications. For other applications requiring far-red phalloidins, we recommend CF®633, CF®640R, or CF®680R phalloidins, which have more stable binding.

Note: CF®555 Phalloidin (00040, 00040-T) has been discontinued. We recommend CF®568 Phalloidin as a replacement for conventional microscopy, or CF®583R Phalloidin as a replacement for STORM.

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

Download a list of CF® dye Phalloidin references.

 

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