F4002-10mg | Phos binding reagent (Phosbind) acrylamide Clinisciences

Description

Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.

The determination of the phosphorylation state of proteins is important for defining protein kinase substrates and revealing the activation state of signal transduction pathways. Methods for determining the phosphorylation status of proteins are thus have important implications in the understanding of diverse biological and pathophysiological processes, such as signal transduction pathways, cancer and other diseases.

Phos binding reagents (Phosbind) are specific and selective phosphate-binding reagents, and exhibit preferential ionic interactions with phosphorylated ions on phosphorylated proteins or peptides at neutral pH. Phos binding reagent is a dinuclear metal (Zn²⁺ or Mn²⁺) complex.

Phosbind Acrylamide is a specific reagent for separation of phosphorylated proteins using SDS-PAGE method.

Product Citation

1. Meng-Yu Liu, Li-Xiao Sun, et al. "De NovoPurine Nucleotide Biosynthesis Pathway Is Required for Development and Pathogenicity inMagnaporthe oryzae." J Fungi (Basel). 2022 Aug 29;8(9):915. PMID: 36135640
2. Xiaofei Liu, Chunyan Zang, et al. "Homeodomain-interacting protein kinase HIPK4 regulates phosphorylation of manchette protein RIMBP3 during spermiogenesis." J Biol Chem. 2022 Aug 2;102327. PMID: 35931115
3. Jian Huang, Luxin Wang, et al. "CDC-like kinase 4 deficiency contributes to pathological cardiac hypertrophy by modulating NEXN phosphorylation." Nat Commun. 2022 Jul 30;13(1):4433. PMID: 35907876
4. Huilin Li, Jiali Ou, et al. "ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude." Commun Biol. 2022 Jul 28;5(1):750. PMID: 35902736
5. Gang Xiang, Shuxuan Wang, et al. "UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer." Cell Death Dis. 2022 May 12;13(5):451. PMID: 35551175
6. Guangli Wang, Chan Fan, et al. "Type VI secretion system-associated FHA domain protein TagH regulates the hemolytic activity and virulence of Vibrio cholerae." Gut Microbes. 2022 Jan-Dec;14(1):2055440. PMID: 35383540
7. Zhao-Huan Zhang, Jian-Mei Xiong, et al. "WNK3-PER1 interactions regulate the circadian rhythm in the suprachiasmatic nucleus in rats." Am J Transl Res. 2022 Feb 15;14(2):1001-1009. PMID: 35273702
8. Qing Ye, Yingying Zhang, et al. "The important roles and molecular mechanisms of annexin A2 autoantibody in children with nephrotic syndrome." Ann Transl Med. 2021 Sep;9(18):1452. PMID: 34734004
9. Joseph O Magliozzi, James B Moseley. "Pak1 kinase controls cell shape through ribonucleoprotein granules." Elife. 2021 Jul 20;10:e67648. PMID: 34282727
10. Yamin Meng, Xing Zhang, et al. "A Novel Nitrogen and Carbon Metabolism Regulatory Cascade Is Implicated in Entomopathogenicity of the Fungus Metarhizium robertsii." mSystems. 2021 Jun 29;6(3):e0049921. PMID: 34156296
11. Li kaihuai, Gaoge Xu, et al. "A predatory soil bacterium reprograms a quorum sensing signal system to regulate antifungal weapon production in a cyclic-di-GMP-independent manner." Research Square. March 11th, 2021.
12. Haijing Guo, Jen-Hsuan Wei, et al. "Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation." J Cell Sci. 2021 Feb 19;134(4):jcs258356. PMID: 33526712
13. Amina Merwa LEKEHAL. "altFUS, the second protein encoded in the FUS gene, inhibits mitochondrial recruitment of PARKIN." Mars, 2021.
14. Fan XJ, Wang YL, et al. "NONO phase separation enhances DNA damage repair by accelerating nuclear EGFR-induced DNA-PK activation." Am J Cancer Res. 2021 Jun 15;11(6):2838-2852. PMID: 34249431
15. Wirth M, Mouilleron S, et al. "Phosphorylation of the LIR Domain of SCOC Modulates ATG8 Binding Affinity and Specificity." J Mol Biol. 2021 Jun 25;433(13):166987. PMID: 33845085
16. Zhixiao Liu, Liujun Wang, et al. "Heterogeneous Responses to Mechanical Force of Prostate Cancer Cells Inducing Different Metastasis Patterns." Adv. Sci. 2020, 1903583.
17. Chengcheng Wang, Taiping Zhang, et al. "Metformin inhibits pancreatic cancer metastasis caused by SMAD4 deficiency and consequent HNF4G upregulation." Protein & Cell (2020);31 July 2020. PMID: 32737864
18. Wen-Jie Shu, Runfa Chen, et al. "Rph1 coordinates transcription of ribosomal protein genes and ribosomal RNAs to control cell growth under nutrient stress conditions." Nucleic Acids Res. 2020 Sep 4;48(15):8360-8373. PMID: 32619236
19. Haijing Guo, Jen-Hsuan Wei, et al. "TPX2 activation by GM130 controls astral microtubule formation and spindle orientation." bioRxiv. March 17, 2020.
20. Domagoj Bareti, Michael Jenkyn-Bedford, et al. "Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork." Mol Cell. 2020 Apr 29;S1097-2765(20)30254-9. PMID: 32369734
21. Huang CY, Gonzalez-Lopez C, et al. "hipBA toxin-antitoxin systems mediate persistence in Caulobacter crescentus." Sci Rep. 2020;10(1):2865. PMID: 32071324
22. Li Zhang, Ruci Wang, et al. "Separable regulation of POW1 in TAF2-mediated grain development and BR-mediated leaf angle formation in rice." bioRxiv. 2019 November 04.
23. Jordann Alyse Smak. "Cell Cycle-Specific Functions and Regulation of the DNA Nuclease SNM1B." University of Michigan. 2019.
24. Xie Y, Lv X, et al. "HPD degradation regulated by the TTC36-STK33-PELI1 signaling axis induces tyrosinemia and neurological damage." Nat Commun. 2019 Sep 19;10(1):4266. PMID: 31537781
25. Jing ZF, Bi JB, et al. "miR-19 promotes the proliferation of clear cell renal cell carcinoma by targeting the FRK-PTEN axis." Onco Targets Ther. 2019 Apr 10;12:2713-2727. PMID: 31043790
26. Wang B, Kettenbach AN, et al. "The Phospho-Code Determining Circadian Feedback Loop Closure and Output in Neurospora." Mol Cell. 2019 Mar 26. pii: S1097-2765(19)30177-7. PMID: 30954403
27. Yu J, Deng T, et al. "Structural basis for protein phosphatase 1 recruitment by glycogen-targeting subunits." FEBS J. 2018 Dec;285(24):4646-4659. PMID: 30422398
28. Yin Y, Wu S, et al. "The MAPK kinase BcMkk1 suppresses oxalic acid biosynthesis via impeding phosphorylation of BcRim15 by BcSch9 in Botrytis cinerea." PLoS Pathog. 2018 Sep 13;14(9):e1007285. PMID: 30212570
29. Sauvé V, Sung G, et al. "Mechanism of parkin activation by phosphorylation." Nat Struct Mol Biol. 2018 Jul;25(7):623-630. PMID: 29967542

Hide ↑↑

Features

1. Recognition of all phosphorylated forms of Tyr/Ser/Thr.

2. Followed by Western blotting and Mass analysis.

3. Simultaneous detection of phosphorylated/non-phosphorylated proteins using the total antibody without the anti phosphorylated protein.

4. Simply add Phos binding reagent acrylamide and MnCl2 solution* to acrylamide solution in the preparation of SDS-PAGE gel.

(*: Add 5.0 mM Phos binding reagent Acrylamide Aqueous Solution and 10 mM MnCl2 solution in equal amount in the gel).

References

1. M. J. Hubbard, P. Cohen. On target with a new mechanism for the regulation of protein phosphorylation. Trends Biochem. Sci. 18: 172 (1993).

2. B. Agnew et al. Compositions and methods for detection and isolation of phosphorylated molecules. US Patent # 7,102,005. (September 5, 2006).

Chemical Properties

Storage	2-10℃
M.Wt	594.71
Formula	C₃₃H₃₈N₈O₃
Solubility	>29.7mg/mL in DMSO
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.