Qualitative
Phosphate standard curve in 96-well plate assay. 100 uL PiBlueTM Reagent was added to 50 uL phosphate standard. After 30 minute incubation, the OD at 620 nm was measured on a plate reader.Useful detection range was 0.4 uM to 50 uM phosphate. The detection limit estimated from blank control values was 20 pmoles. Coefficient of was performed using a competitor's product.
- Specifications
Product Description
Phosphate Assay Kit for sensitive and high-throughput phosphate determination.
Available Test
500 tests
Detection Method
OD 620 nm (malachite green)
Limit of Detection
0.4 uM
Regulatory Status
For research use only (RUO)
Quality Control Testing
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store at 4°C. Avoid multiple freeze-thaw cycles.
- Applications
Qualitative
Phosphate standard curve in 96-well plate assay. 100 uL PiBlueTM Reagent was added to 50 uL phosphate standard. After 30 minute incubation, the OD at 620 nm was measured on a plate reader.Useful detection range was 0.4 uM to 50 uM phosphate. The detection limit estimated from blank control values was 20 pmoles. Coefficient of was performed using a competitor's product.