Specifications

Product Data
C-Tag	mGFP
E. coli Selection	Chloramphenicol (34 ug/mL)
Mammalian Cell Selection	Puromycin
Features	This Lenti vector is a third generation lentiviral vector. All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal monomeric GFP tag. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli selection: Chloramphenicol (34ug/ml) V2_F Primer can be used to sequence the target sequence after cloning. mGFP_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites