PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx™
Cat# 31037-X
Size : 1kit
Brand : Biotium
PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)
A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.
Product Description
This kit is designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the Z3276 gene.
Features
- Selectively detect viable E. coli O157:H7 using qPCR
- Contains primers to amplify the Z3276 gene
- Works even in complex samples or mixed cultures
- Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
- Sufficient reagents for 200 PCR reactions
Kit Components
- PMA dye or PMAxx™ dye
- PMA Enhancer for Gram Negative Bacteria
- Forget-Me-Not™ EvaGreen® qPCR Master Mix
- ROX reference dye
- Z3276 primer mix, 5 uM
About this kit
E. coli O157:H7 is a pathogenic strain of Escherichia coli bacteria. Often a food-borne pathogen, it can cause digestive illness. PMA-based viability PCR to detect E. coli O157:H7 has been reported using the primers provided in the kit, and ORF Z3276 was shown to be specific for strain O157:H7 . These primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix. For pan-E. coli primers to detect other strains of E. coli besides O157:H7, please see kit 31050.
To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.
Strain-Specific Bacterial Viability PCR Kits
| Bacteria Strain | Gene name | Kit with PMA (catalog #) | Kit with PMAxx™ (catalog #) |
|---|---|---|---|
| Salmonella enterica | invA | 31033 | 31033-X |
| Mycobacterium tuberculosis | groEL2 | 31034 | N/A |
| Staphylococcus aureus | nuc | 31035 | N/A |
| MRSA | mecA | 31036 | N/A |
| E. coli O157:H7 | Z3276 | 31037 | 31037-X |
| E. coli | uidA | 31050 | 31050-X |
| Listeria monocytogenes | hly | 31051 | 31051-X |
| Legionella pneumophila | mip | 31053 | N/A |
Don't see your favorite strain? Let us know at [email protected].
Technology
The Full Spectrum Blog
Product Attributes
Size
Viability dye
Storage Conditions
Assay type/options
Detection method/readout
Documents, Protocols, SDS and COA
COA
Request the Certificate of AnalysisReferences
Download PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.