Orb347816-1mg | Rat IgA (alpha chain) Antibody Alkaline Phosphatase

Rat IgA (alpha chain) Antibody Alkaline Phosphatase Conjugated

SKU: orb347816

Rat IgA (alpha chain) antibody (Alkaline Phosphatase)

Signal Transduction

−

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Reactivity	Rat
Application Notes	This product has been assayed against 1.0 ug of Rat IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 is suggested for this product.

−

Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Rat IgA alpha heavy chain
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgA and Rat Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat or human heavy or light chain isotypes.
Conjugation	AP

−

Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	1.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

−

Goat anti-Rat IgA (alpha chain) Alkaline Phosphatase Conjugated Antibody, Goat anti-Rat IgA alpha Antibody Alkaline Phosphatase Conjugation

Western Blot (IB, immunoblot)

IHC

Immunohistochemistry

ELISA

Enzyme-linked Immunosorbent Assay (EIA)