Rat IgE (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, WB
Recommended Dilution	This antibody can be used: • As unlabelled secondary antibody for indirect detection of IgE in Rat cell, tissue substrates and body fluids. • In Immunofluorescence and immunoenzyme assay methods. • For the production of immunoconjugates with a selected marker. • To prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. • As catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. Recommended Dilutions: Histochemistry and Cytochemistry: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Reactivities	Rat
Host	Goat
Isotype	IgG
Clonality	Polyclonal
Immunogen	Purified homogenous IgE isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and Double Radial Immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with Rat IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in Rat serum. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail because of the low level of IgE in most species. Performance Testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of Rat cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Formulation	PBS, pH 7.2 without preservatives. State: Azide Free State: Lyophilized purified IgG fraction.
Form	Azide Free
Reconstitution Method	Restore by adding 1 ml of sterile distilled water.
Concentration	10 mg/ml
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation	Unconjugated
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. Special attention is given to the elimination of antibodies to the common Fab portion of immunoglobulins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.