Rat IgM (mu chain) Antibody Biotin Conjugated

Cat# orb347836-1mg

Size : 1mg

Brand : Biorbyt


Rat IgM (mu chain) Antibody Biotin Conjugated

SKU: orb347836

Description

Rat IgM (mu chain) antibody (Biotin)

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
ReactivityRat
Application Notes
Anti-Rat IgM Biotin conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenRat IgM whole molecule
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
ConjugationBiotin

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Goat Anti Rat IgM (mu chain) Antibody Biotin Conjugated, Goat Anti-Rat IgM mu chain Antibody Biotin Conjugation

Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol