96 Tests PN: A311117

Components:

45x Ab-conjugated beads (S4P12 - Rat IL-2 Ab-bead). PN: A311117A. One vial containing 100 µL of anti-rat IL-2 conjugated to AimPlex Bead S4P12.

25x Biotin-detection Ab (Rat IL-2 Biotin-dAb). PN:A311117B. One vial containing 100 µL of biotinylated anti-rat IL-2.

Lyophilized Standard Mix - Rat Group 1 Panel A, 7-Plex. PN: RG10007. One vial containing lyophilized recombinant rat IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying Rat IL-2. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

Description:

Interleukin 2 (Accession P17108) is a member of the interleukin cytokine family secreted primarily by activated CD4+ and CD8+ T-cells, NK cells, and dendritic cells. IL-2 participates in immune responses but also in delivering signals for thymic development, which in turn promotes homeostasis of T-cells. IL-2 promotes T-cell differentiation into effector, regulatory, or memory T-cells. Elevated levels of IL-2 may be correlated with blood hypereosinophilia, hemophagocytic lymphohistiocytosis, and a range of cardiopulmonary toxicities such as tachycardia and dyspnea.

References:

  1. Hayden A, Lin M, Park S, Pudek M, Schneider M, Jordan MB, Mattman A, Chen LYC. Soluble interleukn-2 receptor is a sensitive diagnostic test in adult HLH. Blood Advances. 2017; 1(26): 2529-2534. Doi: 10.1182/bloodadvances.2017012310

  2. Letourneau S, van Leeuwen EMM, Krieg C, Martin C, Pantaleo G, Sprent J, Surh CD, Boyman O. Il-2/anti-IL-2 antibody complexes show strong biological activity by avoiding interaction with IL-2 receptor α subunit CD25. PNAS. 2010; 107(5): 2171-2176. Doi:10.1073/pnas.0909384107.

  3. Liao W, Lin JX. Leonard WJ. IL-2 family cytokines: new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation. Curr. Opin. Immunology. 2011; 23 (5): 598-604. Doi:10.1016/j.coi.2011.08.003.

  4. Malek TR, Castro I. Interleukin-2 Receptor Signaling: At the Interface between Tolerance and Immunity. Immunity. 2010; 33 (2): 153-165. Doi: 10.1016/j.immuni.2010.08.004.

  5. Prin L, Plumas J, Gruart V, Loiseau S, Aldebert D, Ameisen JC, Vermersch A, Fenaux P, Bletry O, Capron M. Elevated Serum Levels of Soluble Interleukin-2 Receptor: A Marker of Disease Activity in the Hypereosinophilic Syndrome. Blood Journal. 1991; 78: 2626-2632. ISSN: 1528-0020.