FAH-STRM-1-48 | RayPlex® Human Cytokine Storm Bead Array (25-Plex)

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
bFGF Gene Symbol: FGF2 FGFB Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 SCYA11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin ELISA	GCSF Gene Symbol: CSF3 C17orf33 GCSF Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GM-CSF Gene Symbol: CSF2 GMCSF Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA
IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p70 Gene Symbol: IL12A NKSF1 Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	IL-13 Gene Symbol: IL13 NC30 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	IL-17A Gene Symbol: IL17A CTLA8 IL17 Gene ID: 3605 Accession #: Q16552 ELISA Kit: RayBio Human IL-17 ELISA
IL-1 beta (IL-1 F2) Gene Symbol: IL1B IL1F2 Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-1 Ra (IL-1 F3) Gene Symbol: IL1RN IL1F3 IL1RA Gene ID: 3557 Accession #: P18510 ELISA Kit: RayBio Human IL-1 RA ELISA	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA
IL-6 Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA	IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 MCP1 SCYA2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 G0S19-1 MIP1A SCYA3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA
MIP-1 beta (CCL4) Gene Symbol: CCL4 LAG1 MIP1B SCYA4 Gene ID: 388372 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	PDGF-BB Gene Symbol: PDGFB PDGF2 SIS Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	RANTES (CCL5) Gene Symbol: CCL5 D17S136E SCYA5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum/Plasma Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix (Item 5) can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

Size	48 tests, 96 tests, 192 tests, 480 tests
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	25
Solid Support	Fluorescent Microbeads
Design Principle	Sandwich-based
Method Of Detection	Flow Cytometry
Research Area	Inflammation, Infectious Disease, COVID-19
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.

RayPlex® Human Cytokine Storm Bead Array (25-Plex)

Cat# FAH-STRM-1-48

Brand : RayBiotech

RayPlex^® Human Cytokine Storm Bead Array (25-Plex)

Product Description

Specifications

Product Features

Target Names

Application Notes

documents

citations

Multi-omics analysis of host airway responses in pediatric Mycoplasma pneumoniae pneumonia reveals potential mechanisms of disease exacerbation caused by co-infection

Novel mediator in anaphylaxis: decreased levels of miR-375-3p in serum and within extracellular vesicles of patients

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