Recombinant Human R-SPONDIN-1
Cat# 100-208-100UG
Size : 100ug
Brand : FUJIFILM
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Phone : +1 850 650 7790
Recombinant Human R-Spondin-1
R-Spondin 1 (RSPO-1) is a secreted growth factor that stabilizes β-catenin and enhances WNT signaling. RSPO-1 is expressed in many tissues and regulates female sex determination, XX gonad development, oocyte differentiation, and hematopoietic stem cell specification. The RSPO-1 stabilization of β-catenin promotes intestinal crypt cell proliferation. This suggests that RSPO-1 functions as a stem cell growth factor and may have therapeutic potential in the treatment of gastrointestinal diseases and chemotherapy-induced intestinal mucositis. Additionally, recombinant RSPO-1 is a critical protein used to establish 3D intestinal organoid cultures.
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Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | hRspo1, Roof plate-specific spondin-1 |
| Accession Number | Q2MKA7 |
| Source | Genetically modified CHO cells |
| Predicted Molecular Mass | Monomer, 26.8 kDa (243 aa) |
| AA Sequence | SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing phosphate buffered saline (PBS) |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL (50% confidence) | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a | ≤ 50 ng/mL |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.