Figure 1 ELISA Test Antibodies: SARS-CoV Spike Antibody (1 ug/mL). A sandwich ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.
Liquid. SARS-CoV Spike antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Conjugation
Unconjugated
Application
Enzyme-linked immunosorbent assay
Reconstitution and Storage
Stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Immunogen
Anti-SARS-CoV Spike antibody (3225) was raised against a peptide corresponding to 15 amino acids near the center of SARS-CoV Spike glycoprotein. The immunogen is located within amino acids 650-700 of SARS-CoV Spike.
Purification
Affinity Purified
Predicted Homology Based on Immunogen Sequence
Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 40%, homology 56%)
Concentration
1 mg/ml
Blocking Peptide
For anti-S (OAPB00218) antibody is Catalog # OEPB02312
Application Info
SARS-CoV Spike antibody can be used for the detection of SARS-CoV Spike protein in ELISA. It will detect 5 ng of free peptide at 1 ug/mL.
Reference
Marra et al. Science 2003;300:1399-404. Rota et al. Science 2003;300:1394-9. Navas-Nartin et al. J Neurovirol. 2004;10:75-85. Li et al. Nature 2003;426:450-4.
Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
What is the species homology for "SARS-CoV Spike Antibody (OAPB00218)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "SARS-CoV-2 / COVID-19 Virus|Severe Acute Respiratory Syndrome Coronavirus|Virus".
How long will it take to receive "SARS-CoV Spike Antibody (OAPB00218)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
What buffer format is "SARS-CoV Spike Antibody (OAPB00218)" provided in?
This item is provided in "Liquid. SARS-CoV Spike antibody is supplied in PBS containing 0.02% sodium azide.". Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "SARS-CoV Spike Antibody (OAPB00218)"?
This target may also be called "E2;Peplomer protein;sars2;spike glycoprotein." in publications.
What is the shipping cost for "SARS-CoV Spike Antibody (OAPB00218)"?
The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.
What is the guarantee for "SARS-CoV Spike Antibody (OAPB00218)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "SARS-CoV Spike Antibody (OAPB00218)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "". Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "SARS-CoV Spike Antibody (OAPB00218)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "S"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "S"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "S"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "S"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "S"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "S"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
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