STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (AlexaFluor®647)

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Additionally, Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 via the MAPK or mTOR pathway (8,9). Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3alpha (86kD) and Stat3beta (79kD) depend on cell type, ligand exposure or maturation stage of the cells (10). It is notable that Stat3beta lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: 1:50, Add 10ul of the conjugated antibody to 500,000 cells in 90ul PBS/0.5% BSA
Optimal dilutions to be determined by the researcher.

May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing Alexa Fluor® conjugates will result in a substantial loss of activity. Alexa Fluor® conjugates are sensitive to light.

Mab

IgG

7H65(D3A7)

rabbit

mouse

Not determined

Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-5.

Purified prior to conjugation.

Synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3. Species sequence homology

Recognizes endogenous levels of mouse Stat3 only when phosphorylated at Tyr705. Does not crossreact with phospho-EGFR or with corresponding phospho-tyrosines of other Stat proteins. Species Crossreactivity

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.