Stenabolic (SR9009)
Cat# M5143-10mg
Size : 10mg
Brand : AbMole Bioscience
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All AbMole products are for research use only, cannot be used for human consumption.
Stenabolic; REV-ERB Agonist II
Quality Control & Documentation
Biological Activity
Stenabolic (SR9009) is an agonist of REV-ERB, with IC50 of 670 nM and 800 nM for REV-ERBα and REV-ERBβ respectively. Stenabolic (SR9009) inhibits the activity of the SCN clock, with reversible inhibition of circadian oscillations in SCN explants cultured from the Per2:luc reporter mouse. Stenabolic (SR9009) dose-dependently increases the REV-ERB-dependent repressor activity assessed in HEK293 cells expressing a chimeric Gal4 DNA Binding Domain (DBD) - REV-ERB ligand binding domain (LBD) α or β and a Gal4-responsive luciferase reporter. Stenabolic (SR9009) potently and efficaciously suppresses transcription in a cotransfection assay using full-length REV-ERBα along with a luciferase reporter driven by the Bmal1 promoter (SR9009 IC50=710 nM).
Customer Product Validations & Biological Datas
 | Source | Cell Physiol Biochem (2018). Figure 4. SR9009 |
| Method | MTT assay | |
| Cell Lines | The human monocytic leukemia cell line | |
| Concentrations | 1, 5, 10, and 50 µM | |
| Incubation Time | 12 h | |
| Results | Expression of hMMP-9 mRNA also decreased when cells were treated with SR9009 alone, without Ang II stimulation (P < 0.05). The effect of SR9009 was further confirmed on mouse MMP-9 (mMMP-9) mRNA expression in RAW264.7 macrophages |
Protocol (for reference only)
| Cell Experiment | |
|---|---|
| Cell lines | L929 cell |
| Preparation method | Cells were treated with 100 ng/ml IFNg (Millipore) and 10 ng/ml LPS(Millipore) for M1 polarization. M2 differentiation was induced by incubating macrophages with 15 ng/ml IL-4 (Millipore). |
| Concentrations | 10 μM |
| Incubation time | 24 h |
| Animal Experiment | |
|---|---|
| Animal models | homozygous LDL receptor deficient (ldlr−/−) male mice |
| Formulation | PBS |
| Dosages | 100 mg/kg |
| Administration | |
Chemical Information
| Molecular Weight | 437.94 |
| Formula | C20H24ClN3O4S |
| CAS Number | 1379686-30-2 |
| Solubility (25°C) | DMSO ≥ 60 mg/mL |
| Storage | Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
References
[1] Geldof L, et al. Int J Mol Sci. In Vitro Metabolic Studies of REV-ERB Agonists SR9009 and SR9011.
[2] Solt LA, et al. Nature. Regulation of circadian behaviour and metabolism by synthetic REV-ERB agonists.