tdTomato antibody

Cat# orb182397-100ug

Size : 100ug

Brand : Biorbyt

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Phone : +1 850 650 7790

tdTomato antibody

Catalog Number: orb182397

Catalog Numberorb182397
CategoryAntibodies
DescriptionGoat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-Fr, IHC-P, WB
ReactivityOther
IsotypeIgG
ImmunogenPurified recombinant peptide produced in E. coli.
Concentration1mg/ml
Dilution rangeWB 1:500-1:2,000
IHC (F) 1:50-1:500
IHC (P) 1:50-1:500
IF 1:50-1:500
Form/Appearance​Polyclonal antibody supplied in PBS, 20% glycerol and 0.05% sodium azide
PurityThis antibody is epitope-affinity purified from goat antiserum
ConjugationUnconjugated
MW55 kDa
TargettdTomato
Protein SequenceMEQKLISEEDLGTISTMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK
StorageFor continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours
Buffer/PreservativesPBS, 20% glycerol and 0.05% sodium azide
Alternative namesCherry fluorescent protein, DsRed, mCherry, red fl
NoteFor research use only
Application notesIn 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein)
Expiration Date12 months from date of receipt.
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