TNNT2 ELISA Kit (Rabbit)
Cat# OKCD01747
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for TNNT2 ELISA Kit (Rabbit) (OKCD01747) |
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| COA Datasheet | Click Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Predicted Species Reactivity | Oryctolagus cuniculus|Rabbit | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 2, Cardiac (TNNT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin T Type 2, Cardiac (TNNT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T Type 2, Cardiac (TNNT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Troponin T Type 2, Cardiac (TNNT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 15.62-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin T Type 2, Cardiac (TNNT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin T Type 2, Cardiac (TNNT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 6.2 pg/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Troponin T Type 2, Cardiac (TNNT2). No significant cross-reactivity or interference between Troponin T Type 2, Cardiac (TNNT2) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
| Gene Symbol | TNNT2 |
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| Alias Symbols | Cardiac muscle troponin T. |
| NCBI Gene Id | 100009428 |
| Protein Name | Troponin T, cardiac muscle |
| Description of Target | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Uniprot ID | P09741 |
| Protein Accession # | XP_017203358.1 |
| Nucleotide Accession # | XM_017347869.1 |
| Protein Size (# AA) | 301 |