Total Cytotoxicity Test BioAssay™ Kit
Cat# 474046-250T
Size : 250Tests
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
Applications
FCShipping Temp
Blue IceStorage Temp
4°C/-20°CSample Type:
Cell culture
Reagent Name:
7-AAD, CFSE, SR-VAD-FMK
Target:
Cell-mediated cytotoxicity, poly caspases
Excitation/Emission:
7-AAD–546nm/647nm; CFSE–492nm/520nm; SR-FLICA–565nm/600nm
Method of Analysis:
Flow cytometry
Intended Use:
Total Cytotoxicity & Apoptosis Assay is a single-tube, tri-color assay for quantitative assessment of cell-mediated cytolytic activity due to apoptosis and necrosis. The assay employs a green fluorescent cellular stain, CFSE, to label target cells, the red live/dead viability dye, 7-AAD, to identify the dead cells present in the cytotoxicity assay samples, and the orange-red SR-FLICA reagent, SR-VAD-FMK, to measure caspase activity in the target cell population. Analyze your results using a flow cytometer.
Test Principle:
Cytolytic activity is an important process for eliminating intracellular pathogens and cancer cells. Total Cytotoxicity BioAssay™ allows you to assess cytolytic activity in cell culture.
The Total Cytotoxicity & Apoptosis Assay includes three fluorescent reagents: CFSE, 7-AAD, and SR-FLICA. CFSE, a green fluorescing cellular stain, is utilized to identify the target cell population. The unstained effector cells are added and incubated with the target cells. SR-FLICA reagent is incubated with cells to identify those containing active caspases. 7-AAD, a red fluorescing live/dead stain, is then added to stain all necrotic cells red by binding to the DNA of membrane-compromised cells. Through flow cytometry, quantify four populations of cells: live, early apoptotic, late apoptotic, and necrotic.
Kit Components:
125 tests:
SR-FLICA Reagent (SR-VAD-FMK), 1 vial
CFSE target cell stain, 1 vial
7-AAD vital stain, 1 vial
10X Assay Buffer, 1x30mL
SR-FLICA Reagent (SR-VAD-FMK), 1 vial
CFSE target cell stain, 1 vial
7-AAD vital stain, 1 vial
10X Assay Buffer, 1x30mL
250 tests:
SR-FLICA Reagent (SR-VAD-FMK), 2 vials
CFSE target cell stain, 1 vial
7-AAD vital stain, 2 vials
10X Assay Buffer, 1x60mL
CFSE target cell stain, 1 vial
7-AAD vital stain, 2 vials
10X Assay Buffer, 1x60mL
Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards