Transfection Reagent (in vitro)
Cat# MBS635268-1mL
Size : 1mL
Brand : MyBiosource
EGFP expression in cell lines transfected with T8198-03.|(A) primary mouse dendritic cells derived from bone marrow
EGFP expression in cell lines transfected with T8198-03.|(B) HeLa cells
Comparison of T8198-03 with tranfection reagents. HeLa cells were transfected with plasmid DNA encoding eGFP. Transfections were performed according to manufacturers' recommendations and analysed by Flow Cytometry.
Transfection Reagent Reagent – in vitro
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635268MBS635268 Transfection Reagent (in vitro) is a sterile solution of a cationic polymer in water. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery into eukaryotic… More details
Product Overview
Product Name
Transfection Reagent
Full Product Name
Transfection Reagent (in vitro)
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Reagents Required but Not Supplied
Serum-free DMEM, RPMI or other growth medium.
The presence of antibiotics in the medium has no effect on transfection efficiency.
The presence of antibiotics in the medium has no effect on transfection efficiency.
General Considerations DNA Quality Requirements
DNA quality is critical for successful transfection. An A260/A280 ratio of 1.8 or higher is recommended. Endotoxin-contaminated DNA may result in inefficient transfection and cause unacceptably high cellular toxicity
Cell Density
The recommended confluency for adherent cells at the day of transfection is 70-90%. Suspension cells should be plated at an optimal density ensuring their logarithmic growth at the time of transfection.
Incubation Time
Transient transgene expression takes place within 2-72 hours after transfection. The optimal incubation time depends on the cell type, promoter strength and expression product, and must be determined experimentally.
Choice of Promoter
High transfection efficiency depends both on the transgene promoter and on the cell line used. Cytomegalovirus (CMV) promoter is commonly used for high gene expression in a variety of cell lines. Other promoters, such as those from simian virus (SV40) and from Rous sarcoma virus (RSV) can also be used.
Transfection Reagent/DNA Ratio
The volume of transfection reagent used depends on the amount of DNA, transgene and cells to be transfected. The ratios presented in the protocols below are starting amounts and can be further optimized for best results.
Transfection in the Presence of Serum
The transfection efficiency with MBS635268 Transfection Reagent (in vitro) is equally high in the presence of serum. This is not the case with many other transfection reagents.
Antibiotics
Antibiotics do not interfere with both DNA/TurboFect complex formation and cell transfection.
Quality Control
Transfection efficiency was tested on HeLa cells using 1ug of eGFP expressing plasmid and 2ul of TurboFect per 5x10e4 cells in 24-well plate. Transfection efficiency, i.e. the percentage of transfected cells, is 90±10% as estimated by Flow Cytometry.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic applications.
Product Categories/Family for Transfection Reagent reagent
Other Notes
Small volumes of Transfection Reagent reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Transfection Reagent purchase
MBS635268 is a reagent targeting Transfection Reagent. Also known as Transfection Reagent (in vitro).
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