Ankyrin R Antibody

Cat# ASM10319-P594-100

Size : Onrequest

Brand : Abcepta

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  • ICC/IF - Ankyrin R Antibody ASM10319
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin R Monoclonal Antibody, Clone S388A-10 (ASM10319). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin R Monoclonal Antibody (ASM10319) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Ankyrin R Antibody (D) Composite.
  • WB - Ankyrin R Antibody ASM10319
    Western Blot analysis of Rat Brain showing detection of ~200 kDa Ankyrin-R protein using Mouse Anti-Ankyrin-R Monoclonal Antibody, Clone S388A-10 (ASM10319). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Ankyrin-R Monoclonal Antibody (ASM10319) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession P16157
Other Accession NP_000028.3
Host Mouse
Isotype IgG2B
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human Ankyrin R Monoclonal IgG2B
Target/Specificity Detects ~200kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.
Other Names Ankyrin-R Antibody, Ankyrin-1 Antibody, Erythrocyte Ankyrin Antibody, ANK-1 Antibody, ANK Antibody, rCG_43073 Antibody
Clone Names S388A-10
Immunogen Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:100 dilution of SMC-487 was sufficient for detection of Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cytoskeleton | Myofibril | Sarcomere | M line