ASM10316-P594-100 | beta 4 Spectrin Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2 (ASM10316). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody (ASM10316) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) beta 4 Spectrin Antibody (D) Composite.
Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2 (ASM10316). Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody (ASM10316) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF
Primary Accession	Q9H254
Other Accession	NP_066022.2
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Human beta 4 Spectrin Monoclonal IgG1
Target/Specificity	Detects ~>200kDa. Does not cross react with other Beta-spectrins
Other Names	BetaIV-spectrin sigma1 Antibody, Non-erythrocytic spectrin beta chain 4 Antibody, Non-erythroidspectrin beta chain 3 Antibody, SPTBN4 Antibody, SPTBN3 Antibody, Spnb4 Antibody, KIAA1642 Antibody, EMBL BAB83243.1 Antibody, Ensembl ENSRNOP00000028393 Antibody
Clone Names	S393-2
Immunogen	Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:100 dilution of SMC-484 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasm \| Cytoskeleton \| Cytoplasm \| Cell Cortex

beta 4 Spectrin Antibody