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> GluN1/NR1 Antibody

GluN1/NR1 Antibody

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Cat# ASM10244-P594-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - GluN1/NR1 Antibody ASM10244
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN1/NR1 Monoclonal Antibody, Clone S308-48 (ASM10244). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody (ASM10244) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GluN1/NR1 Antibody (D) Composite.
  • WB - GluN1/NR1 Antibody ASM10244
    Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone S308-48 (ASM10244). Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody (ASM10244) at 1:1000.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession P35439
Other Accession NP_058706.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GluN1/NR1 Monoclonal IgG1
Target/Specificity Detects ~105kDa.
Other Names NMDAR1 Antibody, NMDA Receptor 1 Antibody, NMDAR Antibody, NMDA1 Antibody, GRIN1 Antibody, Glutamate [NMDA] receptor subunit zeta-1 Antibody, Glutamate receptor ionotropic N methyl D aspartate 1 Antibody, MRD8 Antibody, N methyl D aspartate receptor Antibody, N methyl D aspartate receptor channel subunit zeta 1 Antibody, N methyl D aspartate receptor subunit NR1 Antibody, NMD-R1 Antibody, NMDA 1 Antibody, NMDA R1 Antibody, NMDZ1_HUMAN Antibody, NR1 Antibody, GluN1 Antibody
Clone Names S308-48
Immunogen Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-410 was sufficient for detection of NR1 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Cell Junction | Synapse | Postsynaptic Cell Membrane