ASM10211-P594-100 | Kv3.4 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (ASM10211). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (ASM10211) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive, stains nucleoli .
Western Blot analysis of Rat brain membrane lysate showing detection of Kv3.4 Potassium Channel protein using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (ASM10211). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (ASM10211) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (ASM10211). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (ASM10211) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hints of filaggrin-like staining.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF, IP, AM
Primary Accession	Q63734
Other Accession	NP_001116248.1
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Rat Kv3.4 Monoclonal IgG1
Target/Specificity	Detects ~70kDa (100kDa in brain due to glycosylation).
Other Names	Raw3 Antibody, Voltage-gated potassium channel subunit Kv3.4 Antibody, Potassium voltage-gated channel subfamily C member 4 Antibody
Clone Names	S72-16
Immunogen	Synthetic peptide amino acids 175-192 of rat Kv3.4
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-335 was sufficient for detection of Kv3.4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Membrane

Kv3.4 Antibody