ASM10035-P594-100 | PSD95 Antibody Clinisciences

Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 (ASM10035). Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (ASM10035) at 1:1000.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 (ASM10035). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (ASM10035) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.
Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 (ASM10035). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (ASM10035) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining.
Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 (ASM10035). Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (ASM10035) at 1:1000.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF, AM
Primary Accession	P31016
Other Accession	NP_062567.1
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat, Bovine
Clonality	Monoclonal
Description	Mouse Anti-Rat PSD95 Monoclonal IgG1
Target/Specificity	Detects ~100kDa. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.
Other Names	PSD 95 Antibody, PSD-95 Antibody, DLG4 Antibody, SAP90 Antibody, Synapse-associated protein 90 Antibody, Postsynaptic density protein 95 Antibody, Disks large homolog 4 Antibody
Clone Names	7E3
Immunogen	Recombinant rat PSD-95
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.
Cellular Localization	Cell Membrane \| Cell Junction \| Synapse \| Postsynaptic Cell Membrane \| Postsynaptic Density \| Cell Projection \| Axon

PSD95 Antibody