SHANK3 Antibody

Cat# ASM10212-P594-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

  • IHC - SHANK3 Antibody ASM10212
    Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (ASM10212). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (ASM10212) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.
  • ICC/IF - SHANK3 Antibody ASM10212
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (ASM10212). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (ASM10212) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive.
  • WB - SHANK3 Antibody ASM10212
    Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (ASM10212). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (ASM10212) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP, AM
Primary Accession Q9JLU4
Other Accession NP_067708.1
Host Mouse
Isotype IgG2b
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat SHANK3 Monoclonal IgG2b
Target/Specificity Detects ~190kDa. No cross-reactivity against Shank1 or Shank2.
Other Names AI841104 antibody, DEL22q13.3 antibody, KIAA1650 antibody, Proline rich synapse associated protein 2 antibody, Proline-rich synapse-associated protein 2 antibody, ProSAP2 antibody, PSAP2 antibody, SH3 and multiple ankyrin repeat domains 3 antibody, SH3 and multiple ankyrin repeat domains protein 3 antibody SH3/ankyrin domain gene 3 antibody, SHAN3_HUMAN antibody, Shank postsynaptic density protein antibody, Shank3 antibody, Shank3b antibody, SPANK 2 antibody, SPANK2 antibody
Clone Names S69-46
Immunogen Synthetic peptide amino acids 840-857 of rat Shank3
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-336 was sufficient for detection of Shank3 in 10 µg COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cell Junction | Synapse | Postsynaptic Cell Membrane | Postsynaptic Density