Amniotic Fluid Stem Cell Culture

Stem cells obtained from the amniotic fluid have recently become an interesting alternate source of pluripotent stem cells for therapeutic applications. Similar to embryonic stem cells, amniotic fluid stem cells are clonogenic and have the capacity to differentiate into cells of the endoderm, mesoderm and ectoderm lineages. These cells are characterized by their positive expression of Oct4-A, SOX2 and NANOG. PRIME-XV™ AFSC Expansion Medium is the first commercially available medium specifically designed to expand this small subset of cells in the amniotic fluid. 

Human Amniotic Fluid Stem Cell Expansion Medium

PRIME-XV™ AFSC Expansion Medium (Cat. 91133)

  • Supports robust expansion of human amniotic fluid stem cells (AFSCs)
  • Maintains pluripotent characteristic of human AFSCs in extended culture
  • Manufactured under cGMP conditions
  • Available in 250mL complete component and ready-to-use
  • Custom packaging available



Amniotic Fluid Stem Cell Preservation

PRIME-XV™ Hypothermic Preservation Solution (Cat. 31003)

  • Protein-Free, Defined Hypothermic Preservation Solution for Cells and Tissues
  • Animal component-free, protein-free solution
  • Supports storage and shipping stability for biologics under hypothermic (2–8°C) conditions
  • Reduces cellular stress response from chilling and re-warming of cells and tissues
  • Provides pH buffering, osmotic/oncotic support and energy substrates to balance intracellular states at low temperatures
  • No additional components are needed
  • Manufactured under cGMP conditions
  • Available in 10 mL packaging


PRIME-XV™ Cryogenic Preservation Solution (Cat. 31004)

  • Protein-Free, Defined Cryogenic Preservation Solution for Cells and Tissues
  • Animal component-free, protein-free solution
  • Enables a variety of cell types to preserve at -80°C to -196°C temperature environments
  • Reduces post-preservation necrosis and apoptosis compared to commercial and home-brew isotonic formulations
  • No additional components are needed
  • Manufactured under cGMP conditions
  • Available in 10 mL packaging