FlexLISA® Kits

FlexLISA® Kits

Flexible, quick and easy ELISA development and optimization!

One Step ELISA protocole - Fast conjugation with your antibody of choice

The FlexLISA® kits are designed for the development and optimization of sandwich ELISA assays to detect the presence of any antigen with a high affinity for any antibody pair in complex samples (serum, plasma, urine etc.).

Benefits of the FlexLISA® kit:
  • Easy antibody labeling – uses world leading Lightning-Link® technology
  • Rapid
  • Uses 40 times less capture antibody – save on cost of materials
  • Use your antibodies of choice
  • Conjugate up to 3 capture and 3 detection antibodies – ideal for screening
  • Flexibility to run multiple conjugates on a plate simultaneously
  • Choice of HRP or AP for enzymatic detection

A rapid one-step protocol is used in this kit, employing pre-blocked microtitre well strips coated with biotin. The FlexLISA® assay is run by adding the sample and the antibody mix to the wells, incubating for 1 hour, washing and reading the assay plate. FlexLISA® is ideal for quick and reliable ELISA assay development, antibody pair screening and ELISA assay optimization.

The kit contains:
  • Lightning-Link® conjugation technology for the labeling of the capture and detection antibodies of choice
- Lightning-Link Streptavidin (3x10ug*) – for labeling up to 3 different capture antibodies
- Lightning-Link HRP or alkaline phosphatase (3x10ug*) – for conjugation of up to 3 different detection antibodies
  • A biotin pre-coated 96 well stripwell plate which can be provided either as clear or black, depending on the enzymatic substrate (12 x 8 -strip well) in a re-sealable foil pouch
Not provided: antibodies, assay buffers, enzyme detection solutions

Enzyme Plate type Size Product code
HRP 96 well clear stripwell plate 1 plate 4200-0010
96 well black stripwell plate 1 plate 4200-0030
AP 96 well clear stripwell plate  1 plate 4200-0020
96 well black stripwell plate 1 plate 4200-0040