Lectins for glycoprotein research

Agarose bound lectins

Lectin affinity chromatography is a simple and widely used technique for the isolation of a variety of glycoconjugates.

This coupling method provides several advantages over the traditional cyanogen bromide procedure: Maximum carbohydrate binding activity of the coupled lectins is retained Linkage is stable over a range of pH values Conjugated proteins are not leached off the beads by Tris or other routinely used buffers No residual charges are present after conjugation minimising non-specific binding to the matrix.

Labeled Lectins

Some lectins can tolerate a higher degree of conjugation and still remain fully active, soluble, and retain low nonspecific binding properties, while others cannot. Each of our labelled lectins has an appropriate number of bound fluorochromes or biotins , providing optimum staining characteristics for each lectin. These conjugates are supplied essentially free of unconjugated fluorochromes or biotins, preserved with sodium azide.

Specificity Guide for lectins

Sugar	Lectin
Fucose	AAL, LTL, UEA I
Galactose	ACL, ECL, GSL I,Jacalin, MAL I, PNA, RCA I, RCA II, SBA
Glucose	Con A, LCA, PSA
Mannose	Con A, GNL, HHL, LCA, NPL, PSA
N-Acetylgalactosamine	BPL, DBA, GSL I, MPL, RCA I, RCA II, SBA, VVA, WFA
N-Acetylglucosamine	DSL, GSL II, LEL, STL, WGA
Sialic Acid	MAL II, SNA
Complex Structures	PHA-E, PHA-L