Mycoplasma detection for reliable cell culture results

Mycoplasma contamination represents one of the most insidious and widespread threats in mammalian cell culture. These wall-less bacteria are invisible under standard microscopy, do not produce turbidity or pH shifts, and can profoundly alter cellular behavior without obvious signs.

Mycoplasma can affect gene expression, growth rates, metabolism, chromosome stability, and experimental reproducibility. In biopharmaceutical production and research laboratories, undetected contamination compromises data integrity, wastes valuable resources, and may jeopardize regulatory compliance.

Routine, sensitive screening is therefore essential. CliniSciences provides a complete portfolio of advanced detection solutions — from ultra-rapid isothermal tests to high-performance qPCR kits — enabling fast, reliable, and user-friendly Mycoplasma detection directly from culture supernatant.

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Featured Detection Solutions

Ultra-Rapid Isothermal Detection

Flip Mycoplasma delivers results in only 12 minutes with minimal hands-on time (<1 minute). This isothermal assay offers a limit of detection of 25 CFU/mL, targets the 16S rRNA gene, and covers >95% of common contaminants. No DNA extraction required – direct input from supernatant with visual readout on an integrated test strip.

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Reliable Endpoint PCR Detection

MycoDect™ Mycoplasma Detection Kit provides highly sensitive endpoint PCR results in under two hours. It targets the most prevalent species responsible for the majority of contaminations and includes all necessary reagents for simple, robust testing using cell lysates or culture media. Results are clearly visualized via standard agarose gel electrophoresis.

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qPCR Mycoplasma Detection Kits – Gold Standard for Routine Screening

Our comprehensive range includes Venor®GeM qEP / qOneStep, TransDetect® Direct qPCR, SAFENSURE™ Rapid qPCR, and other high-performance kits. These enable sensitive, quantitative detection directly from culture supernatant in under 2 hours, making them ideal for high-throughput routine monitoring and quality control.

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Why Routine Mycoplasma Screening Matters

Undetected Mycoplasma contamination can invalidate months of research by altering cellular physiology and experimental outcomes. Regular testing with sensitive, validated methods is a cornerstone of good cell culture practice and ensures data reliability, reproducibility, and compliance with quality standards in both academic and industrial settings.

CliniSciences solutions combine speed, sensitivity, and ease of use, allowing laboratories to implement efficient monitoring protocols without disrupting workflows.

Secure Your Research Integrity Today

Equip your laboratory with fast, reliable, and highly sensitive Mycoplasma detection tools. From ultra-rapid isothermal assays to gold-standard qPCR solutions, CliniSciences offers the complete range needed for effective contamination control.

Browse All Mycoplasma Detection Kits

If you need technical advice, protocol recommendations, or assistance selecting the most suitable kit for your laboratory, please contact our scientific support team at tech@clinisciences.com.