30% OFF: Boost Expression, Maximize Yield
Proteins are the molecular engines of life, driving every biological process from cellular signaling to immune defense. Producing them on demand through recombinant expression systems has become a cornerstone of modern drug discovery, vaccine development, and diagnostics.
A 30% discount is now available on a selected range of insect and mammalian expression tools.
Key Products
Whether scaling up culture volumes or engineering viral vectors, this comprehensive range of high-aeration flasks, baculovirus expression systems, insect and mammalian host cell lines, and chemically defined media is designed to deliver consistent, scalable, and high-yield protein production across the most demanding research and bioproduction applications.
Cell Culture Vessels & Accessories›
- Up to 10× increased oxygenation vs standard flasks.
- Low-shear baffle design with 0.2 µm vented cap.
- 125 mL to 7 L formats for seamless scale-up
- ESF 921™: serum & protein-free, Sf9 densities up to 15×10⁶ cells/mL.
- ESF AdvanCD™: 100% chemically defined, animal-origin-free, cGMP compliant.
- Up to 2× higher cell growth vs. competing insect cell media.
- Pre-adapted to serum-free suspension, ready to use.
- Sf9, Sf21, Tni for BEVS; HEK-293 & CHO for mammalian expression.
- Available in ESF 921, ESF AF, and ESF SFM formulations.
Expression Vectors & Baculovirus DNA›
- Highly efficient recombination with all polyhedrin-based transfer vectors.
- v-cath/chiA deletion eliminates damaging proteases.
- ORF1629 deletion ensures only recombinant virus propagates.
- Expres2 TR optimized specifically for insect cell cotransfection.
- Virus titers several logs higher than Grace's medium.
- All-in-one cotransfection kits for simplified virus generation.
- Production Boost Additive enhances recombinant protein yields.
- Virus Stabilization Additive preserves baculovirus stock integrity.
- Fully compatible with all ESF media formulations.
- Flow cytometric confirmation of baculovirus infection.
- Direct measure of transfection efficiency and virus titer.
- Compatible with all Expression Systems baculovirus workflows.
Application Note
A joint application note from Expression Systems and Thomson Instrument Company demonstrates that ESF AdvanCD™ medium and Optimum Growth® flasks deliver reproducible cell growth and protein yields across 125 mL to 5 L scales — confirming a trusted, scalable platform from research to production.
Figure 1 (A) Cell Density & Viability: Sf9 cells expanded from 2.5×10⁶ to 5.95×10⁶ cells/mL (doubling time: 19.2h) prior to infection at MOI 3. Culture viability at study termination was 71.8% (50 mL) and 83.7% (200 mL), reflecting the lytic nature of baculovirus infection.
Figure 1(B): SEAP Expression — Protein yields were equivalent across both culture volumes, rising from ~107 mg/L at 48h to ~179 mg/L at 72 hours post-infection, with successful infection confirmed by an increase in average cell diameter from 16.6 to 19.8 µm.
Planning the next protein expression experiment?
The protein expression portfolio is now available at 30% OFF!