SARS-CoV-2 (COVID-19) Nucleocapsid protein
Cat# orb638880-1mg
Size : 1mg
Brand : Biorbyt
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SARS-CoV-2 (COVID-19) Nucleocapsid protein
Catalog Number: orb638880
| Catalog Number | orb638880 |
|---|---|
| Category | Proteins |
| Description | Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool. |
| Reactivity | Virus |
| Tag | N-6×His |
| Form/Appearance | Powder |
| Purity | 90% |
| Conjugation | Unconjugated |
| MW | 49.4 kDa |
| Target | Nucleocapsid protein |
| Protein Sequence | QHO62115.1 |
| Source | SARS-CoV-2 Nucleocapsid protein, His Tag (orb638880) is expressed from E. coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). |
| Expression System | E. coli |
| Expression Region | Met 1 - Ala 419 |
| Endotoxins | 1.0 EU per μg |
| NCBI | QHO62115.1 |
| Storage | -20℃ |
| Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 |
| Alternative names | Nucleocapsid protein,NP,Protein N |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 49.4 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE). |
| Expiration Date | 6 months from date of receipt. |
Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.3 ng/mL (QC tested).
SARS-CoV-2 Nucleocapsid protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.