SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active)

Cat# GTX02774-pro-100ug

Size : 100ug

Brand : Genetex

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SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active). GTX02774-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active). GTX02774-pro

Application

Functional Assay

Species

SARS Coronavirus 2
Package
100 μg
View product citations for antibody GTX02774-pro on CiteAb

APPLICATION

Application Note

Binding assay: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 (COVID-19) Spike Protein at 2μg/ml (100 μl/well) can bind recombinant human ACE2 protein with a linear range of 0.15-3.72 ng/mL.

Observed MW

180 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

C-terminal 6 x His tagged ; Val11-Gln1208 (S1+ S2 extracellular domain) of SARS-COV-2 spike protein (Accession #YP_009724390.1).

Expression System

HEK293 cells

Purity

> 95% by SDS-PAGE

Endotoxin

< 0.1 EU /μg determined by LAL method

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SARS-CoV-2 Spike , COVID-19 Spike

Database

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active). GTX02774-pro

GTX02774-pro Image

SDS-PAGE analysis of GTX02774-pro SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active). GTX02774-pro

GTX02774-pro Functional Assay Image

Functional ELISA analysis of immobilized GTX02774-pro SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active) at 2μg/mL (100 μl/well) can bind recombinant human ACE2 protein with a linear range of 0.15-3.72 ng/mL.

Application Reference

Yuan-Chieh Yeh et al. Front Pharmacol 2021; 12 : 765553 Honeysuckle (Lonicera japonica) and Huangqi (Astragalus membranaceus) Suppress SARS-CoV-2 Entry and COVID-19 Related Cytokine Storm in Vitro.
Yuan-Chieh Yeh et al. et al. Front Pharmacol. 2021; 12 (765553) Honeysuckle (Lonicera japonica) and Huangqi (Astragalus membranaceus) Suppress SARS-CoV-2 Entry and COVID-19 Related Cytokine Storm in Vitro

REVIEW

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Application Reference
Yuan-Chieh Yeh et al. Front Pharmacol 2021; 12:765553 Honeysuckle (Lonicera japonica) and Huangqi (Astragalus membranaceus) Suppress SARS-CoV-2 Entry and COVID-19 Related Cytokine Storm in Vitro.
Yuan-Chieh Yeh et al. et al. Front Pharmacol. 2021; 12 (765553) Honeysuckle (Lonicera japonica) and Huangqi (Astragalus membranaceus) Suppress SARS-CoV-2 Entry and COVID-19 Related Cytokine Storm in Vitro