SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active)
Cat# GTX02774-pro-100ug
Size : 100ug
Brand : Genetex
Application | Functional Assay |
---|---|
Species | SARS Coronavirus 2 |
Package | 100 μg |
View product citations for antibody GTX02774-pro on CiteAb
APPLICATION
Application Note
Binding assay: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 (COVID-19) Spike Protein at 2μg/ml (100 μl/well) can bind recombinant human ACE2 protein with a linear range of 0.15-3.72 ng/mL.
Observed MW
180 kDa.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Region/Sequence
C-terminal 6 x His tagged ; Val11-Gln1208 (S1+ S2 extracellular domain) of SARS-COV-2 spike protein (Accession #YP_009724390.1).
Expression System
HEK293 cells
Purity
> 95% by SDS-PAGE
Endotoxin
< 0.1 EU /μg determined by LAL method
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SARS-CoV-2 Spike , COVID-19 Spike
Database
DATA IMAGES
GTX02774-pro ImageSDS-PAGE analysis of GTX02774-pro SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active). |
GTX02774-pro Functional Assay ImageFunctional ELISA analysis of immobilized GTX02774-pro SARS-CoV-2 (COVID-19) Spike (ECD) Protein, His tag (active) at 2μg/mL (100 μl/well) can bind recombinant human ACE2 protein with a linear range of 0.15-3.72 ng/mL. |
Application Reference
Yuan-Chieh Yeh et al. Front Pharmacol 2021; 12 : 765553 Honeysuckle (Lonicera japonica) and Huangqi (Astragalus membranaceus) Suppress SARS-CoV-2 Entry and COVID-19 Related Cytokine Storm in Vitro.
Yuan-Chieh Yeh et al. et al. Front Pharmacol. 2021; 12 (765553) Honeysuckle (Lonicera japonica) and Huangqi (Astragalus membranaceus) Suppress SARS-CoV-2 Entry and COVID-19 Related Cytokine Storm in Vitro
REVIEW
Application Reference
Yuan-Chieh Yeh et al. Front Pharmacol 2021; 12:765553 Honeysuckle (Lonicera japonica) and Huangqi (Astragalus membranaceus) Suppress SARS-CoV-2 Entry and COVID-19 Related Cytokine Storm in Vitro.
Yuan-Chieh Yeh et al. et al. Front Pharmacol. 2021; 12 (765553) Honeysuckle (Lonicera japonica) and Huangqi (Astragalus membranaceus) Suppress SARS-CoV-2 Entry and COVID-19 Related Cytokine Storm in Vitro