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> HSP60 Antibody

HSP60 Antibody

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Cat# ASM10016-STR-200

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - HSP60 Antibody ASM10016
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (ASM10016). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20°C for 10 minutes. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.
  • IHC - HSP60 Antibody ASM10016
    Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (ASM10016). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
  • WB - HSP60 Antibody ASM10016
    Western Blot analysis of Human Cell line lysates showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (ASM10016). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • IHC - HSP60 Antibody ASM10016
    Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (ASM10016). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
  • ICC/IF - HSP60 Antibody ASM10016
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK1, (ASM10016). Tissue: skin Fibroblasts. Species: Human. Fixation: Cold 100% methanol for 30 minutes at -20°C . Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:1000 for 1 hour at RT. Secondary Antibody: DAKO LSAB2 streptavidin-peroxidase system. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain. Courtesy of: Valentina di Felice, University of Palermo, Italy.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E, IP
Primary Accession P10809
Other Accession NP_002147.2
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Xenopus, Bovine, Sheep, Guinea Pig, Dog, Drosophila
Clonality Monoclonal
Description Mouse Anti-Human HSP60 Monoclonal IgG1
Target/Specificity Detects ~60kDa.
Other Names CPN60 Antibody, GROEL Antibody, HLD4 Antibody, HSP 60 Antibody, HSP65 Antibody, HSPD1 Antibody, HuCHA60 Antibody, SPG 13 Antibody
Clone Names LK1
Immunogen Recombinant human HSP60
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.05 µg/ml of SMC-110 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.
Cellular Localization Mitochondrion | Mitochondrion Matrix