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> CD74 Antibody

CD74 Antibody

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Cat# ASM10026-STR-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - CD74 Antibody ASM10026
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (ASM10026). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (ASM10026) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus. Endosome. Lysosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CD74 Antibody. (C) Composite.
  • IHC - CD74 Antibody ASM10026
    Immunohistochemistry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (ASM10026). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (ASM10026) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Beautiful basal to suprabasal staining in epidermis, dermis, hair follicles and muscle.
  • WB - CD74 Antibody ASM10026
    Western Blot analysis of Human N87 cell lysates showing detection of CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (ASM10026). Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (ASM10026) at 1:1000. Lysates treated with macrophage inhibitory factor (MIF). Courtesy of: Victor E. Reyes, University of Texas Medical Branch, USA.
  • ICC/IF - CD74 Antibody ASM10026
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (ASM10026). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (ASM10026) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP, FCM, FACS
Primary Accession P04233
Other Accession NP_001020329.1
Host Mouse
Isotype IgG
Reactivity Human, Mouse
Clonality Monoclonal
Description Mouse Anti-Human CD74 Monoclonal IgG
Target/Specificity Detects ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
Other Names DHLAG Antibody, HLA DR gamma Antibody, HLADG Antibody, p33 Antibody, p35 Antibody, Protein 41 Antibody, CD 74 antibody, CD74 antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, CD74 antigen antibody, CD74 molecule antibody, CD74 molecule, major histocompatibility complex, class II invariant chain antibody, CLIP antibody, DHLAG antibody, Gamma chain of class II antigens antibody, HG2A_HUMAN antibody, HLA class II histocompatibility antigen gamma chain antibody, HLA DR antigens associated invariant chain antibody, HLA DR gamma antibody, HLA-DR antigens-associated invariant chain antibody, HLA-DR-gamma antibody, HLADG antibody, HLADR antigens associated invariant chain antibody Ia antigen associated invariant chain antibody, Ia antigen-associated invariant chain antibody, Ia associated invariant chain antibody, Ia gamma antibody, Ii antibody, Invariant polypeptide of major histocompatibility complex class II antigen associated antibody, la-gamma antibody, Major histocompatibility complex class II invariant chain antibody, MHC HLA DR gamma chain antibody, MHC HLA-DR gamma chain antibody, p33 antibody, p35 antibody, Protein 41 antibody
Clone Names PIN.1
Immunogen Human CD74 invariant chain synthetic peptide
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-116 was sufficient for detection of CD74 in 20 µg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.
Cellular Localization Cell Membrane | Endoplasmic Reticulum | Endoplasmic Reticulum Membrane | Golgi Apparatus | Endosome | Lysosome