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> FIH Antibody

FIH Antibody

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Cat# ASM10125-STR-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - FIH Antibody ASM10125
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (ASM10125). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-FIH Antibody. (C) Composite.
  • IHC - FIH Antibody ASM10125
    Immunohistochemistry analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (ASM10125). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All positive.
  • ICC/IF - FIH Antibody ASM10125
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (ASM10125). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-FIH Antibody. (C) Composite.
  • WB - FIH Antibody ASM10125
    Western Blot analysis of Human Cell lysates showing detection of FIH protein using Mouse Anti-FIH Monoclonal Antibody, Clone fih162c (ASM10125). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession Q9NWT6
Other Accession NP_060372.2
Host Mouse
Isotype IgG1
Reactivity Human, Mouse
Clonality Monoclonal
Description Mouse Anti-Human FIH Monoclonal IgG1
Target/Specificity Detects ~45kDa.
Other Names Factor inhibiting HIF1 (hypoxia-inducible factor) Antibody, DKFZp762F1811 Antibody, Factor inhibiting HIF-1 Antibody, Factor inhibiting HIF1 Antibody, FIH 1 Antibody, FIH-1 Antibody, FIH1 Antibody, FLJ20615 Antibody, FLJ22027 Antibody, HIF1AN Antibody, HIF1N_HUMAN Antibody, Hypoxia inducible factor 1 alpha inhibitor Antibody, Hypoxia inducible factor 1 alpha subunit inhibitor Antibody, Hypoxia inducible factor asparagine hydroxylase Antibody, Hypoxia-inducible factor 1-alpha inhibitor Antibody, Hypoxia-inducible factor asparagine hydroxylase Antibody, Peptide aspartate beta dioxygenase Antibody
Clone Names FIH 162c
Immunogen Full length human FIH expressed in E.coli BL21 (DE3) cells
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-182 was sufficient for detection of FIH in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Nucleus