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> PUMA (CT) Antibody

PUMA (CT) Antibody

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Cat# ASM10419-STR-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - PUMA (CT) Antibody ASM10419
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (ASM10419). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (ASM10419) at 1:125 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • WB - PUMA (CT) Antibody ASM10419
    Western blot analysis of Human, Mouse K562 and 3T3 cell lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (ASM10419). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (ASM10419) at 1:1000.
  • ICC/IF - PUMA (CT) Antibody ASM10419
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (ASM10419). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (ASM10419) at 1:125 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • ICC/IF - PUMA (CT) Antibody ASM10419
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA Polyclonal Antibody (ASM10419). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (ASM10419) at 1:100.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q9BXH4
Other Accession NP_001120712.1
Host Rabbit
Reactivity Human, Mouse
Clonality Polyclonal
Description Rabbit Anti-Human PUMA (CT) Polyclonal
Target/Specificity Detects ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
Other Names BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
Immunogen C-terminal amino acids of human PUMA
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 0.02% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-166 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion