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> Alpha A Crystallin Antibody

Alpha A Crystallin Antibody

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Cat# ASM10063-STR-200

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - Alpha A Crystallin Antibody ASM10063
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha A Crystallin Monoclonal Antibody, Clone 1H3.B8 (ASM10063). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha A Crystallin Monoclonal Antibody (ASM10063) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Alpha A Crystallin Antibody (D) Composite.
  • WB - Alpha A Crystallin Antibody ASM10063
    Western Blot analysis of Bovine tissue lysate showing detection of Alpha A Crystallin protein using Mouse Anti-Alpha A Crystallin Monoclonal Antibody, Clone 1H3.B8 (ASM10063). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha A Crystallin Monoclonal Antibody (ASM10063) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. This blot shows absolute specificity as Left: Alpha A Crystallin, Right: Alpha B Crystallin.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, ICC/IF
Primary Accession P02489
Other Accession NP_000385.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat, Bovine
Clonality Monoclonal
Description Mouse Anti-Human Alpha A Crystallin Monoclonal IgG1
Target/Specificity Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Other Names Heat shock protein beta4 Antibody, Acry 1 Antibody, CRYA1 Antibody, CRYAA Antibody, HSPB4 Antibody
Clone Names 1H3.B8
Immunogen Native Alpha Crystallin
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.5 µg/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Cellular Localization Cytoplasm | Nucleus