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> ATG7 Antibody

ATG7 Antibody

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Cat# ASM10499-STR-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - ATG7 Antibody ASM10499
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody (ASM10499). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody (ASM10499) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.
  • WB - ATG7 Antibody ASM10499
    Western blot analysis of Rat brain cell lysates showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody (ASM10499). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain cell lysates. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody (ASM10499) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.
  • WB - ATG7 Antibody ASM10499
    Western blot analysis of Human Cervical Cancer cell lysates (HeLa) showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody (ASM10499). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical Cancer cell lysates (HeLa). Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody (ASM10499) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession O95352
Other Accession NP_001129503.2
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human ATG7 Polyclonal
Target/Specificity Detects 77.9 kDa.
Other Names Autophagy 7S. cerevisiae, homolog of Antibody, APG7 like Antibody, ATG7_HUMAN Antibody, hAGP7 Antibody, Ubiquitin-like modifier-activating enzyme ATG7 Antibody, Autophagy related protein 7 Antibody, Autophagy-related protein 7 Antibody, GSA 7 Antibody, APG7, S. cerevisiae, homolog of Antibody, Ubiquitin-activating enzyme E1-like protein Antibody, Autophagy-related 7 (yeast) Antibody, APG7 autophagy 7 like Antibody, 1810013K23Rik Antibody, APG7 autophagy 7-like (S. cerevisiae) Antibody, ATG12-activating enzyme E1 ATG7 Antibody, ATG7 autophagy related 7 homolog Antibody, Atg7l Antibody, Ubiquitin activating enzyme E1 like protein Antibody, Apg 7 Antibody, APG7L Antibody, DKFZp434N0735 Antibody, ATG 7 Antibody, APG7-like Antibody, GSA7 Antibody, ATG7 autophagy related 7 homolog (S. cerevisiae) Antibody, ATG7 Antibody
Immunogen Synthetic peptide from the N-terminal of Human ATG7
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-610 was sufficient for detection of ATG7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Preautophagosomal Structure