GFAP R416WT Antibody

Cat# ASM10276-STR-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

  • ICC/IF - GFAP R416WT Antibody ASM10276
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP R416WT Monoclonal Antibody, Clone S206B-9 (ASM10276). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GFAP R416WT Monoclonal Antibody (ASM10276) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GFAP R416WT Antibody (D) Composite.
  • WB - GFAP R416WT Antibody ASM10276
    Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone S206B-9 (ASM10276). Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody (ASM10276) at 1:250.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession P14136
Other Accession NP_001124491.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human GFAP R416WT Monoclonal IgG1
Target/Specificity Detects ~50kDa. Does not cross-react with GFAP-R416W or other proteins (based on KO validation results).
Other Names Glial fibrillary acidic protein Antibody, Intermediate filament protein Antibody, Astrocyte Antibody, gfapl Antibody, DKFZp459C0729 Antibody, MGC139638 Antibody, FLJ45472 Antibody, AI836096 Antibody, GFAP Antibody
Clone Names S206B-9
Immunogen Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. >50% identity with other proteins (Vimentin, Desmin and Peripherin).
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-442 was sufficient for detection of GFAP R416WT in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm

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