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> Trap1 Antibody

Trap1 Antibody

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Cat# ASM10152-STR-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - Trap1 Antibody ASM10152
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6 (ASM10152). Tissue: Myoblast cell line C2C12 . Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (ASM10152) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
  • WB - Trap1 Antibody ASM10152
    Western Blot analysis of Human, Rat Human A431 and Rat Brain Membrane cell lysates showing detection of ~75 kDa Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6 (ASM10152). Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Rat lysate, Rat Brain Membrane (RBM). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (ASM10152) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Color Development: TMB solution for 15 min at RT. Predicted/Observed Size: ~75 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q12931
Other Accession NP_057376.2
Host Mouse
Isotype IgG1 Kappa
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human Trap1 Monoclonal IgG1 Kappa
Target/Specificity Detects ~75kDa.
Other Names Heat shock protein 75 Antibody, Heat shock protein 75 kDa Antibody, Heat shock protein 75 kDa mitochondrial Antibody, HSP 75 Antibody, HSP 90L Antibody, HSP75 Antibody, HSP90L Antibody, mitochondrial Antibody, TNF receptor associated protein 1 Antibody, TNFR associated protein 1 Antibody, TNFR-associated protein 1 Antibody, TRAP 1 Antibody, TRAP-1 Antibody, Trap1 Antibody, TRAP1_HUMAN Antibody, Tumor necrosis factor receptor associated protein Antibody, Tumor necrosis factor type 1 receptor associated protein Antibody, Tumor necrosis factor type 1 receptor-associated protein Antibody
Clone Names 3H4-2H6
Immunogen Purified recombinant TRAP1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-207 was sufficient for detection of Trap-1/HSP75 in 20 µg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion