- More Files
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Bevacizumab monoclonal antibody, clone 6C3 (Cat # MAB23088) with Bevacizumab. Coating antigen: Bevacizumab, 1 ug/mL. Anti-Bevacizumab monoclonal antibody, clone 6C3 dilution start from 1000 ng/mL. EC50 = 8.735 ng/mL. While the antibody does not recognize the human IgG (data not shown).
Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Bevacizumab monoclonal antibody, clone 4H1 (Cat # MAB23087) and Anti-Bevacizumab monoclonal antibody, clone 6C3 (Cat # MAB23088) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Bevacizumab monoclonal antibody, clone 6C3 was labeled with Biotin. The sensitivity is less than 78 pg/mL.
Blocking
Anti-Bevacizumab monoclonal antibody, clone 6C3 (Cat # MAB23088) blocks Bevacizumab binding with VEGF165 protein. Coating antigen: VEGF165, 1 ug/mL. Bevacizumab final concentration: 0.1 ug/mL. Anti-Bevacizumab monoclonal antibody, clone 6C3 dilutions start from 10 ug/mL. IC50 = 0.157 ug/mL.
- Specification
Product Description
Mouse monoclonal antibody raised against Bevacizumab.
Target gene is VEGFA.Immunogen
Bevacizumab.
Host
Mouse
Specificity
The product is specific for Bevacizumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody : Anti-Bevacizumab monoclonal antibody, clone 4H1 (Cat # MAB23087).
Form
Lyophilized
Preparation Method
The antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Bevacizumab.
Purification
Protein A purification
Isotype
IgG1, lambda
Recommend Usage
Blocking (0.1-10 ug/mL)
ELISA (0.01-0.1 ug/mL (Direct/Indirect/Inhibitory))
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.157 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Bevacizumab monoclonal antibody, clone 6C3 (Cat # MAB23088) with Bevacizumab. Coating antigen: Bevacizumab, 1 ug/mL. Anti-Bevacizumab monoclonal antibody, clone 6C3 dilution start from 1000 ng/mL. EC50 = 8.735 ng/mL. While the antibody does not recognize the human IgG (data not shown).Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Bevacizumab monoclonal antibody, clone 4H1 (Cat # MAB23087) and Anti-Bevacizumab monoclonal antibody, clone 6C3 (Cat # MAB23088) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Bevacizumab monoclonal antibody, clone 6C3 was labeled with Biotin. The sensitivity is less than 78 pg/mL.Blocking
Anti-Bevacizumab monoclonal antibody, clone 6C3 (Cat # MAB23088) blocks Bevacizumab binding with VEGF165 protein. Coating antigen: VEGF165, 1 ug/mL. Bevacizumab final concentration: 0.1 ug/mL. Anti-Bevacizumab monoclonal antibody, clone 6C3 dilutions start from 10 ug/mL. IC50 = 0.157 ug/mL. - Gene Info — VEGFA
Entrez GeneID
7422Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
- Interactome
- Pathway
- Disease