CSAG1 Protein Lysate

Referencia OCOA03185

embalaje : 20ug

Marca : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for CSAG1 Protein Lysate (OCOA03185)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCSAG1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCSAG1
Gene Full Namechondrosarcoma associated gene 1
Alias Symbolscancer/testis antigen 24.1;cancer/testis antigen CSAGE;cancer/testis antigen family 24, member 1;CSAGE;CT24.1;putative chondrosarcoma-associated gene 1 protein.
NCBI Gene Id158511
Protein NameNM_153478, NP_705611, Q6PB30
Description of TargetHomo sapiens chondrosarcoma associated gene 1 (CSAG1), transcript variant a, mRNA, Putative chondrosarcoma-associated gene 1 protein, putative chondrosarcoma-associated gene 1 protein [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q6PB30
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_705611
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_153478
Molecular Weight8.7 kDa