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> CSF3 Protein Lysate

CSF3 Protein Lysate

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Referencia OCOA00235

embalaje : 20ug

Marca : Aviva Systems Biology

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    Datasheets/ManualsPrintable datasheet for CSF3 Protein Lysate (OCOA00235)
    Product Info
    Predicted Species ReactivityHomo sapiens
    Product FormatLyophilized
    HostHEK293T
    Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
    ::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
    Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
    TagC-MYC/DDK
    DescriptionCSF3 HEK293T cell transient overexpression lysate (as WB positive control)
    Gene SymbolCSF3
    Gene Full Namecolony stimulating factor 3
    Alias SymbolsC17orf33;CSF3OS;filgrastim;GCSF;granulocyte colony-stimulating factor;granulocyte-colony stimulating factor;lenograstim;pluripoietin.
    NCBI Gene Id1440
    Protein NameNM_000759, NP_000750, P09919
    Description of TargetGranulocyte colony-stimulating factor, granulocyte colony-stimulating factor isoform a precursor [Homo sapiens], Homo sapiens colony stimulating factor 3 (CSF3), transcript variant 1, mRNA
    Uniprot IDhttps://www.uniprot.org/uniprot/P09919
    Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000750
    Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000759
    Molecular Weight22.29 kDa