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> GABA-B Receptor 2 Antibody

GABA-B Receptor 2 Antibody

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Referencia ASM10236-STR-100

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  • ICC/IF - GABA-B Receptor 2 Antibody ASM10236
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone S81-2 (ASM10236). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody (ASM10236) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-B Receptor 2 Antibody (D) Composite.
  • WB - GABA-B Receptor 2 Antibody ASM10236
    Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA B Receptor 2 protein using Mouse Anti-GABA B Receptor 2 Monoclonal Antibody, Clone S81-2 (ASM10236). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 µg). . Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-GABA B Receptor 2 Monoclonal Antibody (ASM10236) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession O88871
Other Accession NP_113990.1  
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GABA-B Receptor 2 Monoclonal IgG1
Target/Specificity Detects ~105kDa. No cross-reactivity against GABA(B)R1.
Other Names BcDNA:GH07312 Antibody, CG6706 Antibody, CT20836 Antibody, D GABA[412]R2 Antibody, D Gaba2 Antibody, FLJ36928 Antibody, G protein coupled receptor 51 Antibody, G-protein coupled receptor 51 Antibody, GAB B R2 Antibody, GABA B R2 Antibody, GABA B receptor 2 Antibody, GABA B RECEPTOR Antibody, GABA-B receptor 2 Antibody, GABA-B-R2 Antibody, GABA-BR2 Antibody, GABA[412]R2 Antibody, GABAB R2 Antibody, GABABR 2 Antibody, GABABR2 Antibody, GABB R2 Antibody, GABBR 2 Antibody, GABBR2 Antibody, GABR2_HUMAN Antibody, Gamma aminobutyric acid (GABA) B receptor 2 Antibody, Gamma aminobutyric acid B receptor 2 Antibody, Gamma aminobutyric acid GABA B receptor 2 Antibody, Gamma aminobutyric acid type B receptor subunit 2 Antibody, Gamma-aminobutyric acid type B receptor subunit 2 Antibody, Gb 2 Antibody, Gb2 Antibody, GH07312 Antibody, GPR 51 Antibody, GPR51 Antibody, GPRC 3B Antibody, GPRC3B Antibody, HG 20 Antibody, HG20 Antibody, HRIHFB2099 Antibody, Metabotropic GABA B receptor subtype 2 Antibody, OTTHUMP00000021776 Antibody, OTTHUMP00000063797 Antibody, R2 SUBUNIT Antibody
Clone Names S81-2
Immunogen Fusion protein amino acids 861-912 of rat GABA(B)R2
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-402 was sufficient for detection of GABA(B)R2 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Cell Junction | Synapse | Postsynaptic Cell Membrane